Summary: F-17 S a human faecal isolate, is resistant to the T-series of bacteriophages (i.e. T2 to T7). A T2-sensitive mutant of F-17 S was isolated following acriflavin treatment. This mutant, F-17 S T was found to be sensitive to the entire T-series of phages. F-17 S and F-17 S T did not differ quantitatively in total LPS content. However, analysis of LPS revealed that a large fraction of F-17 S T was devoid of O-side-chains. This accounted for the sensitivity of this strain to bacteriophages T3, T4, and T7. In addition, F-17 S T contained only about half the amount of capsular material contained by F-17 S accounting for the sensitivity of the mutant to bacteriophages T2, T5, and T6. Although the two strains colonized equally well when fed individually to streptomycin-treated mice, when fed simultaneously to streptomycin-treated mice, F-17 S T colonized at a level of about 1 × 10 cells (g faeces), whereas F-17 S colonized at only 1 × 10 cells (g faeces). These studies suggest that bacterial cell surface components modulate the large intestine colonizing ability of F-17 S in the mouse large intestine.


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