Summary: (group ) was grown in Todd-Hewitt broth (36.4 g 1, pH 7.8) in a Braun Fermenter (type B20) to investigate the conditions of optimal bacterial growth and maximal production of CAMP factor. The influence of different gas atmospheres (air, N, CO, and gas mixtures) on growth, CAMP production and chain length of was studied. The organisms grew best in the presence of 2% (w/v) glucose, at pH 6.2, with a constant flow of CO. The number of diplococci and monococci under these conditions reached almost 80% of the total population.


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