1887

Abstract

SUMMARY: Immunization of rabbits or monkeys with walls prepared from by a procedure including extraction with SDS at room-temperature induced antibodies to three antigens (A, B and C) detectable by crossed Immunoelectrophoresis. Antigens A and B have previously been characterized as proteins of molecular weight 29000 and 190000, respectively. Antigen C was characterized as having a molecular weight of 70000 and was purified by immunosorbent affinity chromatography and hydrophobic interaction chromatography. Another wall protein, antigen D, of molecular weight 13000, was extracted from walls with Triton X-100. Immunization of monkeys with walls prepared from cultures of grown at a high ( = 0.5 h) or low ( = 0.05 h) dilution rate in a chemostat showed that only the latter induced protection against dental caries. There was a positive correlation between levels of antibody to antigens A and C and induction of protection and a negative correlation between protection and the level of antibody to antigen B. No antibody to antigen D was detected in protected monkeys and an experiment in which monkeys were immunized with pure antigen D confirmed that it does not induce protection.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-3-865
1983-03-01
2019-12-06
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-3-865
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error