1887

Abstract

Immunization of rabbits or monkeys with walls prepared from by a procedure including extraction with SDS at room-temperature induced antibodies to three antigens (A, B and C) detectable by crossed Immunoelectrophoresis. Antigens A and B have previously been characterized as proteins of molecular weight 29000 and 190000, respectively. Antigen C was characterized as having a molecular weight of 70000 and was purified by immunosorbent affinity chromatography and hydrophobic interaction chromatography. Another wall protein, antigen D, of molecular weight 13000, was extracted from walls with Triton X-100. Immunization of monkeys with walls prepared from cultures of grown at a high ( = 0.5 h) or low ( = 0.05 h) dilution rate in a chemostat showed that only the latter induced protection against dental caries. There was a positive correlation between levels of antibody to antigens A and C and induction of protection and a negative correlation between protection and the level of antibody to antigen B. No antibody to antigen D was detected in protected monkeys and an experiment in which monkeys were immunized with pure antigen D confirmed that it does not induce protection.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-3-865
1983-03-01
2021-08-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/3/mic-129-3-865.html?itemId=/content/journal/micro/10.1099/00221287-129-3-865&mimeType=html&fmt=ahah

References

  1. Aasted B. 1980; Highly purified agarose as stacking gel in sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. Biochemical Journal 189:183–184
    [Google Scholar]
  2. Beighton D., Hayday H. 1982; The stability of Streptococcus mutanspopulations in the dental plaque of monkeys (Macacafascicularis). Journal of Applied Bacteriology 52:191–200
    [Google Scholar]
  3. Bowen W. H., Cohen B., Cole M. F., Colman G. 1975; Immunisation against dental caries. British Dental Journal 139:45–58
    [Google Scholar]
  4. Burnette W. N. 1981; “Western Blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–303
    [Google Scholar]
  5. Closs O., Harboe M., Wassim A. M. 1975; Crossreactions between Mycobacteria I. Crossed immunoelectrophoresis of souble antigens of Mycobacterium lepraemurium and comparison with BCG. Scandinavian Journal of Immunology 4: (suppl. 2) 173–185
    [Google Scholar]
  6. Cohen B., Colman G., Russell R. R. B. 1979; Immunisation against dental caries: further studies. British Dental Journal 147:9–14
    [Google Scholar]
  7. Colman G., Cohen B. 1979; Immunisation of monkeys (Macacafascicularis) with Streptococcus mutans. In Pathogenic Streptococci pp. 214–215 Edited by Parker M. T. Chertsey, Surrey: Reedbooks Ltd;
    [Google Scholar]
  8. Ellwood D. C., Hunter J. R., Longyear V. M. C. 1974; Growth of Streptococcus mutans in a chemostat. Archives of Oral Biology 19:659–664
    [Google Scholar]
  9. Erlich H. A., Cohen S. N., Mcdevitt H. O. 1978; A sensitive radioimmunoassay for detecting products translated from cloned DNA fragments. Cell 13:681–689
    [Google Scholar]
  10. Hardy L., Jacques N. A., Forester H., Campbell L. K., Knox K. W., Wicken A. J. 1981; Effect of fructose and other carbohydrates on the surface properties, lipoteichoic acid production, and extracellular proteins of Streptococcus mutans Ingbritt grown in continuous culture. Infection and Immunity 31:78–87
    [Google Scholar]
  11. Hughes M., Machardy S. M., Sheppard A. J., Woods N. C. 1980; Evidence for an immunological relationshiip between Streptococcus mutans and human cardiac tissue. Infection and Immunity 27:567–588
    [Google Scholar]
  12. Karcher D., Lowenthal A., Thormar H., Noppe M. 1981; Serological identification of viral antigens after electrophoretic transfer. Journal of Immunological Methods 43:175–179
    [Google Scholar]
  13. Kessler S. W. 1981; Use of Protein-A bearing staphylococci for the immunoprecipitation and isolation of antigens from cells. Methods of Enzymology 73:442–458
    [Google Scholar]
  14. Mcghee J. R., Michalek S. M. 1981; Immunobiology of dental caries: microbial aspects and local immunity. Annual Review of Microbiology 35:595–638
    [Google Scholar]
  15. Norrild B., Bjerrum O. J., Vestergaard B. F. 1977; Polypeptide analysis of individual immunoprecipitates from crossed immunoelectrophoresis. Analytical Biochemistry 81:432–441
    [Google Scholar]
  16. O’Farrell P. H. 1975; High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 10:4007–4021
    [Google Scholar]
  17. Peach S. L., Russell R. R. B. 1982; Antigens of Streptococcus mutans cell walls. In Basic Concepts of Streptococci and Streptococcal Diseases pp. 114–116 Edited by Holm S. E., Christensen P. Chertsey, Surrey: Reedbooks Ltd;
    [Google Scholar]
  18. Rosen A., Ek K., Aman P. 1978; Agarose isoelectric focusing of native human immunoglobulin M and α-2-macroglobulin. Journal of Immunological Methods 28:1–11
    [Google Scholar]
  19. Russell M. W. 1979; Purification and properties of a protein surface antigen of Streptococcus mutans. Microbios 25:7–18
    [Google Scholar]
  20. Russell M. W., Bergmeier L. A., Zanders E. D., Lehner T. 1980; Protein antigens of Streptococcus mutans: purification and properties of a double antigen and its protease-resistant component. Infection and Immunity 28:486–493
    [Google Scholar]
  21. Russell M. W., Caldwell J., Lehner T. 1979; Antibody response to Streptococcus mutans antigens during immunisation against dental caries. In Pathogenic Streptococci pp. 117–218 Edited by Parker M. T. Chertsey, Surrey: Reedbooks Ltd;
    [Google Scholar]
  22. Russell R. R. B. 1979a; Cell-wall protein antigens of Streptococcus mutans. In Pathogenic Streptococci pp. 221–222 Edited by Parker M. T. Chertsey, Surrey: Reedbooks Ltd;
    [Google Scholar]
  23. Russell R. R. B. 1979b; Wall-associated protein antigens of Streptococcus mutans. Journal of General Microbiology 114109–115
    [Google Scholar]
  24. Russell R. R. B. 1979c; Purification of Streptococcus mutansglucosyltransferase by polyethylene glycol precipitation. FEMS Microbiology letters 6:197–199
    [Google Scholar]
  25. Russell R. R. B. 1979d; Glucan-binding proteins of Streptococcus mutans serotype c. Journal of General Microbiology 112:197–201
    [Google Scholar]
  26. Russell R. R. B. 1979e; Glycosyltransferases of Streptococcus mutans strain Ingbritt. Microbios 23:135–146
    [Google Scholar]
  27. Russell R. R. B. 1980; Distribution of cross-reactive antigens A and B in Streptococcus mutans and other oral streptococci. Journal of General Microbiology 118:383–388
    [Google Scholar]
  28. Russell R. R. B. 1981; Non-specific binding of Streptococcus mutans glucan-binding proteins and lipoteichoic acid to immunosorbent columns. FEMS Microbiology Letters 11:279–282
    [Google Scholar]
  29. Russell R. R. B., Beighton D. 1982; Specificity of natural antibodies reactive with Streptococcus mutans in monkeys. Infection and Immunity 35:741–744
    [Google Scholar]
  30. Russell R. R. B., Colman G. 1981; Immunization of monkeys (Macacofascicularis) with purified Streptococcus mutans glucosyltransferase. Archives of Oral Biology 26:23–28
    [Google Scholar]
  31. Russell R. R. B., Beighton D., Cohen B. 1982; Immunisation of monkeys (Macacafascicularis) with antigens purified from Streptococcus mutans. British Dental Journal 152:81–84
    [Google Scholar]
  32. Steinbuch M., Audran R. 1969; The isolation of IgG from mammalian sera with the aid of caprylic acid. Archives of Biochemistry and Biophysics 134:279–284
    [Google Scholar]
  33. Swanstrom R., Shank P. R. 1978; X-ray intensifying screens greatly enhance the detection by autoradiography of the radioactive isotopes 32P and 125I. Analytical Biochemistry 86:184–192
    [Google Scholar]
  34. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Science of the United States of America 76:4350–4354
    [Google Scholar]
  35. Wenham D. G., Hennessey T. D., Cole J. A. 1979; Regulation of glucosyl- and fructosyltransferase synthesis by continuous cultures of Streptococcus mutans. Journal of General Microbiology 114:117–124
    [Google Scholar]
  36. Wrigley C. W. 1971; Gel electrofocusing. Methods in Enzymology 22:559–564
    [Google Scholar]
  37. Yokogawa K., Kawata S., Takemura T., Yoshimura Y. 1975; Purification and properties of lytic enzymes from Streptococcus globisporus 1829. Agricultural and Biological Chemistry 39:1533–1543
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-3-865
Loading
/content/journal/micro/10.1099/00221287-129-3-865
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error