1887

Abstract

LL100-21 grew on acetonitrile, acetamide, a range of aliphatic nitriles and amides, benzonitrile and benzamide as the sources of carbon and/or nitrogen. It also grew on acetate, several other aliphatic acids, and benzoate as carbon sources. Growth on either acetonitrile or acetamide resulted in induction of both acetonitrilase and acetamidase activities, which were shown to be due to separate enzymes. Growth on benzonitrile resulted in the ability to release ammonia from benzonitrile but not benzamide, acetonitrile or acetamide. Growth on benzamide resulted in the ability to form ammonia from benzamide but not benzonitrile, acetonitrile or acetamide. Measurements of respiratory activity of the bacteria grown on the various substrates showed a similar pattern of induction of oxidative activity for these compounds.

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1983-03-01
2024-05-10
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References

  1. Arnaud A., Galzy P., Jallageas J.-C. 1976a; Amidase activity of some bacteria. Folia microbiologica 21:178–184
    [Google Scholar]
  2. Arnaud A., Galzy P., Jallageas J.-C. 1976b; Etude de l’activité nitrilasique de quelques bacteries. Revue des fermentations et des industries alimentaires T.31:39–44
    [Google Scholar]
  3. Arnaud A., Galzy P., Jallageas J.-C. 1977; Etude de l’acétonitrilase d’une souche deBrevi- bacterium. Agricultural and Biological Chemistry 41:2183–2191
    [Google Scholar]
  4. Asano Y., Tani Y., Yamada H. 1980; A new enzyme “nitrile hydratase” which degrades acetonitrile in combination with amidase. Agricultural and Biological Chemistry 44:2251–2252
    [Google Scholar]
  5. Bauchop T., Elsden S. R. 1960; The growth of micro-organisms in relation to their energy supply. Journal of General Microbiology 23:457–469
    [Google Scholar]
  6. Betz J. L., Clarke P. H. 1972; Selective evolution of phenylacetamide-utilizing strains ofPseudomonas aeruginosa. Journal of General Microbiology 73:161–174
    [Google Scholar]
  7. Brammar W. J., Mcfarlane N. D., Clarke P. H. 1966; The uptake of aliphatic amides byPseudomonas aeruginosa. Journal of General Microbiology 44:303–309
    [Google Scholar]
  8. Chamberlain P., Mackenzie R. M. 1981; Enzymatic hydrolysis of nitriles. In Cyanide in Biology pp. 335–348 Edited by Vennesland B., Conn E. E., Knowles C. J., Westley J., Wissing F. London and New York: Academic Press;
    [Google Scholar]
  9. Clarke P. H. 1980; The utilization of amides by microorganisms. In Microorganisms and Nitrogen Sources pp. 537–562 Edited by Payne J. W. New York: John Wiley;
    [Google Scholar]
  10. Digeronimo M. J., Antoine A. D. 1976; Metabolism of acetonitrile and propionitrile byNocardia rhodochrous LL100-21. Applied and Environmental Microbiology 31:900–906
    [Google Scholar]
  11. Fawcett J. K., Scott J. E. 1960; A rapid and precise method for the determination of urea. Journal of Clinical Pathology 13:156–159
    [Google Scholar]
  12. Fukuda Y., Fukui M., Harada T., Izumi Y. 1971; Formation of α-amino acid from a-amino- nitrile by cell suspensions of a strain of Coryne- bacterium. Journal of Fermentation Technology 49:1011–1016
    [Google Scholar]
  13. Fukuda Y., Harada T., Izumi Y. 1973; Formation of L-α-hydroxyacids from DL-α-hydroxynitriles byTorulopsis Candida GN 405. Journal of Fermentation Technology 51:393–397
    [Google Scholar]
  14. Gornall A. G., Bardawill C. J., David M. M. 1949; Determination of serum proteins by the biuret reaction. Journal of Biological Chemistry 177:751–766
    [Google Scholar]
  15. Harper D. B. 1976; Purification and properties of an unusual nitrilase fromNocardia NCIB 11216. Biochemical Society Transactions 4:502–504
    [Google Scholar]
  16. Harper D. B. 1977a; Microbial metabolism of aromatic nitriles. Enzymology of C-N cleavage byNocardia sp.(Rhodochrous group) NCIB 11216. Biochemical Journal 165:309–319
    [Google Scholar]
  17. Harper D. B. 1977b; Fungal degradation of aromatic nitriles. Enzymology of C-N cleavage by Fusar- ium solarti. Biochemical Journal 167:685–692
    [Google Scholar]
  18. Hook R. H., Robinson W. G. 1964; Ricinine nitrilase.II. Purification and properties. Journal of Biological Chemistry 239:4263–4267
    [Google Scholar]
  19. Jallageas J.-C., Arnaud A., Galzy P. 1978; Etude de l’acetamidase d’une souche deBrevibacterium. Journal of General and Applied Microbiology 24:103–114
    [Google Scholar]
  20. Jallageas J.-C., Arnaud A., Galzy P. 1980; Bioconversions of nitriles and their applications. In Advances in Biochemical Engineering 12:1–32
    [Google Scholar]
  21. Kuwahara M., Yanase H., Ishida Y., Kikuchi Y. 1980; Metabolism of aliphatic nitriles inFusarium solani. Journal of Fermentation Technology 58:573–577
    [Google Scholar]
  22. Miller J. H. 1972 Experiments in Molecular Genetics p. 431 Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Robinson W. G., Hook R. H. 1964; Ricinine nitrilase. I. Reaction product and substrate specificity. Journal of Biological Chemistry 239:4257–4262
    [Google Scholar]
  24. Snell F. D., Snell C. T. 1954 Colorimetric Methods of Analysis Including Some Turbidimetric and Nephelometric Methods, 3rd edn. 4 pp. 32–33 Princeton, New Jersey: Van Nostrand;
    [Google Scholar]
  25. Stein W. D. 1967 The Movement of Molecules across Cell Membranes New York: Academic Press;
    [Google Scholar]
  26. Yamada H., Asano Y., Hino T., Tani Y. 1979; Microbial utilization of acrylonitrile. Journal of Fermentation Technology 57:8–14
    [Google Scholar]
  27. Yamada H., Asano Y., Tani Y. 1980; Microbial utilization of glutaronitrile. Journal of Fermentation Technology 58:495–500
    [Google Scholar]
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