1887

Abstract

strains possessing the marker (splitting of the locus and a non-tandem duplication of chromosome segment Ib: ) when transformed or transduced to tryptophan independence mainly give rise to haploid cells with the genetic structure of strain 168. However, among the Trp transformants or transductants about 10% are merodiploid carrying a non-tandem duplication of segment C () while maintaining that of segment Ib. Linkage and segregation studies made it possible to determine their genetic structure, which can be represented by three different maps. In map a the copies of Ib are inverted repeats and one of them is flanked by two direct repeats of segment C; in map two Ib-C segments are inverted repeats and in map the copies of C are inverted repeats with one of them flanked by direct repeats of Ib. It is proposed that transition from map to map and then to map and vice versa, may occur by recombination between inverted repeats of either Ib or C. The merodiploids are unstable, recombination between direct repeats leading to haploid cells of 168-type structure. The models proposed for merodiploid formation call for fusion of two recipient chromosomes mediated by the donor segment and recombination between copies of a DNA sequence of the two chromosomes located in different regions. In the case of PBS-1 mediated transduction the greater length of the donor DNA segment makes it possible to obtain the merodiploids with a single recipient chromosome and this needs only a slight modification of the models. No merodiploids are found in transformation when the recipient carries a deletion of the SP prophage, or in transduction when both donor and recipient possess this deletion. These results indicate that the homologous sequences involved may be part of the SP prophage or that a sequence of bacterial DNA has a good homology with it.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-3-687
1983-03-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/3/mic-129-3-687.html?itemId=/content/journal/micro/10.1099/00221287-129-3-687&mimeType=html&fmt=ahah

References

  1. Anagnostopoulos C. 1977; Genetic analysis of Bacillus subtilis strains carrying chromosomal rearrangements. In Modem Trends in Bacterial Transformation and Transfection pp. 211–230 Portolés A., López R., Espinosa M. Edited by Amsterdam: Elsevier/North-Holland Biomedical Press;
    [Google Scholar]
  2. Anagnostopoulos C., Spizizen J. 1961; Requirements for transformation in Bacillus subtilis. Journal of Bacteriology 81:741–746
    [Google Scholar]
  3. Anagnostopoulos C., Trowsdale J. 1976; Production of merodiploid clones in Bacillus subtilis strains. In Microbiology - 1976 pp. 44–57 Schlessinger D. Edited by Washington, D.C.: American Society for Microbiology.;
    [Google Scholar]
  4. Anderson R. P., Roth J. R. 1977; Tandem genetic duplications in phage and bacteria. Annual Review of Microbiology 31:473–505
    [Google Scholar]
  5. Anderson R. P., Roth J. 1981; Spontaneous tandem genetic duplications in Salmonella typhimurium arise by unequal recombination between rRNA (rrn) cistrons. Proceedings of the National Academy of Sciences of the United States of America 78:3113–3117
    [Google Scholar]
  6. Audit C., Anagnostopoulos C. 1972; Production of stable and persistent unstable heterogenotes in a mutant of Bacillus subtilis. In Spores V pp. 117–125 Halvorson H. O., Hanson R., Campbell L. L. Edited by Washington, D.C.: American Society for Microbiology.;
    [Google Scholar]
  7. Audit C., Anagnostopoulos C. 1973; Genetic studies relating to the production of transformed clones diploid in the tryptophan region of the Bacillus subtilis genome. Journal of Bacteriology 114:18–27
    [Google Scholar]
  8. Audit C., Anagnostopoulos C. 1975; Studies on the size of the diploid region in Bacillus subtilis merozygotes from strains carrying the trpE26 mutation. Molecular and General Genetics 137:337–351
    [Google Scholar]
  9. Fink P. S., Korman R. Z., Odebralski J. M., Zahler S. A. 1981; Bacillus subtilis bacteriophage SPficl is a deletion mutant of SPβ. Molecular and General Genetics 182:514–515
    [Google Scholar]
  10. Henner D. J., Hoch J. A. 1980; The Bacillus subtilis chromosome. Microbiological Reviews 44:57–82
    [Google Scholar]
  11. Hill C. W., Harnish B. W. 1981; Inversions between ribosomal RNA genes of Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 78:7069–7072
    [Google Scholar]
  12. Hill C. W., Grafstrom R. H., Hillman B. S. 1977a; Chromosomal rearrangements resulting from recombination between ribosomal RNA genes. In DNA Insertion Elements, Plasmids and Episomes pp. 497–505 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  13. Hill C. W., Grafstrom R. H., Harnish B. W., Hillman B. S. 1977b; Tandem duplications resulting from recombination between ribosomal RNA genes in Escherichia coli. Journal of Molecular Biology 116:407–428
    [Google Scholar]
  14. Jamet C., Anagnostopoulos C. 1969; Etude d’une mutation tres faiblement transformable au locus de la threonine desaminase de Bacillus subtilis. . Molecular and General Genetics 105:225–242
    [Google Scholar]
  15. Lipsky R. H., Rosenthal R., Zahler S. A. 1981; Defective specialized transducing phages of Bacillus subtilis phage SPβ that carry the sup-3 or sup-44 gene. Journal of Bacteriology 148:1012–1015
    [Google Scholar]
  16. Riley M., Anilionis A. 1978; Evolution of the bacterial genome. Annual Review of Microbiology 32:519–560
    [Google Scholar]
  17. Rosenthal R., Toye P. A., Korman R. Z., Zahler S. A. 1979; The prophage of SPβc2dcitK1, a defective specialized transducing phage of Bacillus subtilis. Genetics 92:721–739
    [Google Scholar]
  18. Schneider A. M., Anagnostopoulos C. 1981; Linkage map and properties of a Bacillus subtilis strain carrying a non-tandem duplication of the purB-tre region of the chromosome. Journal of General Microbiology 125:241–256
    [Google Scholar]
  19. Schneider A. M., Gaisne M., Anagnostopoulos C. 1982; Genetic structure and internal rearrangements of stable merodiploids from Bacillus subtilis strains carrying the trpE26 mutation. Genetics 101:189–210
    [Google Scholar]
  20. Trowsdale J., Anagnostopoulos C. 1975; Evidence for the translocation of a chromosome segment in Bacillus subtilis strains carrying the trpE26 mutation. Journal of Bacteriology 122:886–898
    [Google Scholar]
  21. Trowsdale J., Anagnostopoulos C. 1976; Differences in the genetic structure of Bacillus subtilis strains carrying the trpE26 mutation and strain 168. Journal of Bacteriology 126:609–618
    [Google Scholar]
  22. Warner F. D., Kitos G. A., Romano M. P., Hemphill H. E. 1977; Characterization of SP/L a temperate bacteriophage from Bacillus subtilis 168M. Canadian Journal of Microbiology 23:45–51
    [Google Scholar]
  23. Zahler S. A., Korman R. Z., Rosenthal R., Hemphill H. E. 1977; Bacillus subtilis bacteriophage SPβ localization of the prophage attachment site, and specialized transduction. Journal of Bacteriology 129:556–558
    [Google Scholar]
  24. Zahler S. A., Korman R. Z., Odebralski J. M., Fink P. S., Mackey C. J., Poutre C. G., Lipsky R., Youngman P. J. 1982; Genetic manipulations with phage SPβ. In Molecular Cloning and Gene Regulation in Bacilli pp. 41–50 Ganesan A. T., Chang S., Hoch J. A. Edited by New York: Academic Press.;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-3-687
Loading
/content/journal/micro/10.1099/00221287-129-3-687
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error