1887

Abstract

DNA repair and genetic recombination have been studied in mutants of in which expression of inducible, SOS repair activities was altered by additional mutations either in or and appeared to act additively to increase sensitivity to UV irradiation and to reduce recombination proficiency. The defect was suppressed in strains carrying the allele of but not in strains carrying mutations that increased synthesis of protein or which directly inactivated repressor. These and other data are interpreted to suggest that the defect is related to a reduced activity of protein. The implications of these findings are discussed in relation to the control of SOS activities and cell division during normal growth.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-3-681
1983-03-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/3/mic-129-3-681.html?itemId=/content/journal/micro/10.1099/00221287-129-3-681&mimeType=html&fmt=ahah

References

  1. Armengod M. E., Blanco M. 1978; Influence of therecF mutation ofEscherichia coli K12 on prophage λ induction. Mutation Research 52:37–47
    [Google Scholar]
  2. Bachmann B. J. 1972; Pedigree of some mutant strains ofEscherichia coli K-12. Bacteriological Reviews 36:525–557
    [Google Scholar]
  3. Clark A. J. 1980; A view of the RecB/C and RecF pathways ofE. coli recombination. In Mechanistic Studies of DNA replication and Genetic Recombination (ICN-UCLA Symposium XIX) pp. 891–899 Edited by Alberts B.
    [Google Scholar]
  4. Cunningham R. P., Takehiko S., Dasgupta C., Radding C. M. 1979; Single strands inducerecAprotein to unwind duplex DNA for homologous pairing. Nature, London 28:191–195
    [Google Scholar]
  5. D’Ari R., George J., Huisman O. 1979; Suppression of ^/-mediated induction of SOS functions inEscherichia coli by an altereddnaB protein. Journal of Bacteriology 140:381–387
    [Google Scholar]
  6. George J., Castellazzi M., Buttin G. 1975; Prophage induction and cell division inE. coli. III. MutationssfiA andsfiB restore division intif andIonstrains and permit the expression of mutator properties oftif. . Molecular and General Genetics 140:309–332
    [Google Scholar]
  7. Horii Z., Clark A. J. 1973; Genetic analysis of the RecF pathway to genetic recombination inEscherichia coli K12: isolation and characterization of mutants. Journal of Molecular Biology 80:327–344
    [Google Scholar]
  8. Howe W. E., Mount D. W. 1978; Analysis of cell division in single clones of theEscherichia coli K-12lexA mutant. Journal of Bacteriology 133:1278–1281
    [Google Scholar]
  9. Huisman O., D’Ari R. 1981; An inducible DNA replication-cell division coupling mechanism inE. coli. . Nature, London 290:797–799
    [Google Scholar]
  10. Kato T., Shinoura Y. 1977; Isolation and characterization of mutants ofEscherichia coli deficient in induction of mutation by ultraviolet light. Molecular and General Genetics 156:121–131
    [Google Scholar]
  11. Kenyon C. J., Walker G. C. 1980; DNA- damaging agents stimulate gene expression at specific loci inEscherichia coli. . Proceedings of the National Academy of Sciences of the United States of America 77:2819–2833
    [Google Scholar]
  12. Little J. W., Harper J. E. 1979; Identification of thelex A gene product ofEscherichia coli K-12. Proceedings of the National Academy of Sciences of the United States of America 76:6147–6151
    [Google Scholar]
  13. Little J. W., Edmiston S. H., Pacelli L. R., Mount D. W. 1980; Cleavage of theEscherichia coli lexA protein by therecA protease. Proceedings of the National Academy of Sciences of the United States of America 77:3225–3229
    [Google Scholar]
  14. Lloyd R. G., Barbour S. D. 1974; The genetic location of thesbcA gene ofEscherichia coli. . Molecular and General Genetics 134:157–171
    [Google Scholar]
  15. Lloyd R. G., Low B. 1976; Some genetic consequences of changes in the level ofrecA gene function inEscherichia coli K-12. Genetics 84:675–695
    [Google Scholar]
  16. Mcentee K., Weinstock G. M. 1981; tif-1mutation alters polynucleotide recognition by therecA protein ofEscherichia coli. . Proceedings of the National Academy of Sciences of the United States of America 78:6061–6065
    [Google Scholar]
  17. Mount D. W., Walker A. C., Kosel C. 1973; Suppression oflex mutations affecting deoxyribonucleic acid repair inEscherichia coli K-12 closely linked thermosensitive mutations. Journal of Bacteriology 116:950–956
    [Google Scholar]
  18. Mount D. W., Little J. W., Edmiston S. H. 1980; Influence of plasmids carrying thelexA gene on DNA repair and related processes inEscherichia coli K-12. Molecular and General Genetics 117:477–483
    [Google Scholar]
  19. Phizicky E. M., Roberts J. W. 1981; Induction of SOS functions: regulation of proteolytic activity of E. coli RecA protein by interaction with DNA and nucleoside triphosphate. Cell 25:259–267
    [Google Scholar]
  20. Radding C. M. 1981; Recombination activities ofE. coli RecA protein. Cell 25:3–4
    [Google Scholar]
  21. Ream L. W., Margossian L., Clark A. J., Hansen F. G., Meyenburg K. 1980; Genetic and physical mapping ofrecF inEscherichia coli K-12. Molecular and General Genetics 180:115–121
    [Google Scholar]
  22. Rothman R. H., Clark A. J. 1977; The dependence of postreplication repair onuvrB in arecF mutant ofEscherichia coli K-12. Molecular and General Genetics 155:279–286
    [Google Scholar]
  23. Rothman R. H., Margossian L. J., Clark A. J. 1979; W-reactivation of phage lambda inrecF, recL, uvrA anduvrB mutants ofE. coli K-12. Molecular and General Genetics 169:279–287
    [Google Scholar]
  24. Thomas A., Lloyd R. G. 1980; Altered regulation of therecA gene inEscherichia coli strains carrying arecA-linked suppressor oflexA. . Molecular and General Genetics 179:355–358
    [Google Scholar]
  25. Witkin E. M. 1976; Ultraviolet mutagenesis and inducible deoxyribonucleic acid repair inEscherichia coli. . Bacteriological Reviews 40:869–907
    [Google Scholar]
  26. Zaritsky A., Pritchard R. H. 1973; Changes in cell size and shape associated with changes in the replication time of the chromosome ofEscherichia coli. . Journal of Bacteriology 114:824–837
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-3-681
Loading
/content/journal/micro/10.1099/00221287-129-3-681
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error