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A mutant defective in ornithine carbamoyltransferase activity and having a concomitant aerial mycelium-negative phenotype was isolated from Streptomyces fradiae. The aerial mycelium formation of the mutant could be restored by replacing l-arginine with l-citrulline in the minimal medium. The possibility that the ornithine cycle is connected with the regulation of aerial mycelium formation is discussed.
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