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Rhizobium strain BTAi 1 which was isolated from a stem nodule on Aeschynomene indica was a fast grower with mean generation times of 3·2 and 4·0 h with glucose and mannitol, respectively. Its ability to utilize sucrose and lactose confirmed the similarity of BTAi 1 to other fast growers. However, its inability to utilize rhamnose, dulcitol, raffinose, trehalose, citrate, malate and fumarate linked it to the slow-growing rhizobia. The absence of the pentose phosphate pathway also placed BTAi 1 with the slow-growers as did the presence of 2-keto-3-deoxy-l-arabonate aldolase, its small colony morphology and the production of alkali with most carbon sources. Strain BTAi 1 is thus an intermediate type of Rhizobium, sharing characteristics with both fast and slow growers.
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