1887

Abstract

The specific activity of X-prolyl-dipeptidyl aminopeptidase in grown on glucose-containing medium remains constant during exponential growth and increases less than twofold when cells reach the stationary phase. In cells harvested from exponential growth on glucose-containing medium the specific activity of the enzyme is found to be 20-30% lower than the specific activity observed in media without glucose, containing acetate or ethanol as the carbon source. X-Prolyl-dipeptidyl aminopeptidase is not inactivated after the addition of glucose to stationary phase cells. Growth of the yeast on poor nitrogen sources or under nitrogen-starvation results in a three- to fourfold increase in the level of the enzyme.

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/content/journal/micro/10.1099/00221287-129-11-3519
1983-11-01
2024-04-27
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References

  1. Aibara S., Hayashi R., Hata T. 1971; Physical and chemical properties of yeast proteinase C. Agricultural and Biological Chemistry 35:658–666
    [Google Scholar]
  2. Frey J., Rohm K. 1978; Subcellular localization and levels of aminopeptidases and dipeptidase in Saccharomyces cerevisiae . Biochimica et biophysica acta 527:31–41
    [Google Scholar]
  3. Frey J., Rohm K. 1979; The glucose-induced inactivation of aminopeptidase I inSaccharomyces cerevisiae. . FEBS Letters 100:261–264
    [Google Scholar]
  4. Gancedo J.M., Gancedo C. 1971; Fructose 1,6-diphosphatase, phosphofructokinase and glucose-6-phosphate dehydrogenase from fermenting and nonfermenting yeasts. Archives of Microbiology 76:132–138
    [Google Scholar]
  5. Hansen R.J., Switzer R.L., Hinze H., Holzer H. 1977; Effects of glucose and nitrogen source on the levels of proteinases, peptidases and proteinase inhibitors in yeast. Biochimica et biophysica acta 496:103–114
    [Google Scholar]
  6. Johnston G.C., Singer R.A., Mcfarlane E.S. 1977; Growth and cell division during nitrogen starvation of the yeast Saccharomyces cerevisiae . Journal of Bacteriology 132:723–730
    [Google Scholar]
  7. Ochoa S. 1955; Malic dehydrogenase from pig heart. Methods in Enzymology 1:735–739
    [Google Scholar]
  8. Saheki T., Holzer H. 1975; Proteolytic activities in yeast. Biochimica et biophysica acta 384:203–214
    [Google Scholar]
  9. Suárez Rendueles M.P., Schwencke J., Garciaalvarez N., Gascón S. 1981; A new X-prolyl dipeptidyl aminopeptidase from yeast associated with a particulate fraction. FEBS Letters 131:296–300
    [Google Scholar]
  10. Wolf D.H., Ehmann C. 1978; Carboxypeptidase S from yeast: regulation of its activity during vegetative growth and differentiation. FEBS Letters 91:59–62
    [Google Scholar]
  11. Wolf D.H., Holzer H. 1980; Proteolysis in yeast. In Microorganisms and Nitrogen Sources pp. 431–458 Payne J.W. Edited by Chichester: John Wiley;
    [Google Scholar]
  12. Wolf D.H., Weiser U. 1977; Studies on a carboxypeptidase Y mutant of yeast and evidence for a second carboxypeptidase activity. European Journal of Biochemistry 73:553–556
    [Google Scholar]
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