1887

Abstract

Mutations of the gene on the chromosome result in sensitivity to the dye toluidine blue and, in male cells, cause loss of F pili, producing sterility in conjugation. Compared with its parent, a strain deleted for () showed an altered sensitivity to a wide range of dyes and antibiotics which affect different intracellular processes, and hence it appeared likely that the barrier properties of the cell envelope were impaired. Unlike mutants known to be defective in LPS structure, there appeared to be no correlation between the hydrophobicity of the compounds and the sensitivity of the strain. Moreover there was no difference between and strains in their sensitivity to LPS-specific phages, and chemical and GLC analysis of LPS components revealed no difference between the two strains. Examination of outer and inner membrane proteins from isogenic strains having the deletion and the gene cloned into the plasmid pACYC184, with or without insertional inactivation of by the transposon γδ, was performed by SDS-PAGE. This revealed a number of differences in the profile of proteins from both inner and outer membranes, correlated with mutation in the gene. The gene appears to be identical to the gene, which has been shown to be required for efficient transcription of the sex factor F. It is therefore proposed that the gene product may also control the expression of chromosomal genes coding for envelope proteins.

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1983-11-01
2021-07-24
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