Summary: Extracellular glycosyltransferases from 17 strains of were examined by analytical isoelectric focusing. Three kinds of glucosyltransferase: highly-branched-1,6-α-D-glucan synthetase, 1,3-α-D-glucan synthetase and 1,6-α-D-glucan synthetase, were excreted from serotype and strains. The enzymes of serotype a strains were distinguishable from those of serotypes and by differences in their pI values. Serotype and strains excreted basic glucosyltransferase and acidic fructosyltransferase. Serotype strains also excreted the glucosyl-and fructosyltransferases, but the pI values were different from those of the enzymes from the other serotypes. Thus, strains could be divided into four groups by analytical isoelectric focusing of glycosyltransferases which corresponded well to the four genetic groups.


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