1887

Abstract

Summary: lipopolysaccharide (LPS) preparations lost their receptor properties for phage 1P after deacetylation by alkali. LPS treated with acetylesterase isolated from showed a simultaneous decrease in the amount of acetyl groups and phage receptor activity. The content of -acetyl groups in the LPS of a phage-resistant mutant was significantly decreased when compared to that in LPS from the parent phage-sensitive strain 24SM. Phage 1P could cleave acetyl groups from the extracted LPS without any detectable formation of reducing groups. Binding of phage 1P to the LPS receptor was irreversible. It was demonstrated by electron microscopy that phage DNA was ejected in the presence of LPS from 24SM but not of that from the phage-resistant mutant.

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/content/journal/micro/10.1099/00221287-129-10-3171
1983-10-01
2019-10-15
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-10-3171
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