1887

Abstract

Using 200 fresh isolates of , the relationship between type of growth in soft-agar medium and respiration, dehydrogenase activity and biotype was investigated. When strains of were cultured in Brain Heart Infusion medium containing 0.15% (w/v) agar, the following different growth types were observed: compact colonial morphology with growth throughout the medium (type A), or with growth only at the surface (type B); and diffuse colonial morphology with growth throughout the medium (type C), growth only at the surface (type D), or growth from the surface to the middle of the tube (type E). Five representative strains of each growth type were studied and different results for cytochrome pattern, oxygen consumption and relative activities of lactic dehydrogenase and succinic dehydrogenase were obtained with different growth types. However, there was no correlation between growth type and biotype.

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1982-09-01
2024-05-07
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References

  1. Allen J. 1961; Multiple forms of lactate dehydro-genase in tissues of the mouse; their specificity, cellular localization and response to altered physiological conditions. Annals of the New York Academy of Sciences 94:937–951
    [Google Scholar]
  2. Baird-Parker A. C. 1974; The basis for the present classification of staphylococci and micrococci. Annals of the New York Academy of Sciences 236:7–13
    [Google Scholar]
  3. Collins F. M., Lascelles J. 1962; The effect of growth conditions on oxidative and dehydrogenase activity in Staphylococcus aureus. Journal of General Microbiology 29:531–535
    [Google Scholar]
  4. Englesberg E., Levy J. B., Gibor A. C. 1954; Some enzymatic changes accompanying the shift from anaerobiosis to aerobiosis in Pasteurella pestis. Journal of Bacteriology 68:178–185
    [Google Scholar]
  5. Evans J. B., Kloos W. E. 1972; Use of shake cultures in a semisolid thioglycolate medium for dif-ferentiating staphylococci from micrococci. Applied Microbiology 23:326–331
    [Google Scholar]
  6. Finkelstein R. A., Sulkin S. E. 1958; Characteristics of coagulase negative staphylococci in serumsoft agar. Journal of Bacteriology 75:339–343
    [Google Scholar]
  7. Forsum U., Forsgen A., Hjelum E. 1972; Role of protein A in the serum-soft agar technique. Infection and Immunity 6:583–586
    [Google Scholar]
  8. Frerman F. E., White D. C. 1967; Membrane lipid changes during formation of a functional electron transport system in Staphylococcus aureus. Journal of Bacteriology 94:1868–1874
    [Google Scholar]
  9. Goldenbaum P. E., White D. C. 1974; Role of lipid in the formation and function of the respiratory system of Staphylococcus aureus. Annals of the New York Academy of Sciences 236:115–123
    [Google Scholar]
  10. Gotz F., Schleifer K. H. 1978; Biochemical properties and the physiological role of the fructose 1,6-bisphosphate activated l-lactate dehydrogenase from Staphylococcus epidermidis. European Journal of Biochemistry 90:555–561
    [Google Scholar]
  11. Horan N. J., Midgley M., Dawes E. A. 1978; Anaerobic transport of serine and 2-aminoisobutyric acid by Staphylococcus epidermidis. Journal of General Microbiology 109:119–126
    [Google Scholar]
  12. Howden R. 1976; Use of anaerobic culture for the improved isolation of Streptococcus pneumoniae. Journal of Clinical Pathology 29:50–53
    [Google Scholar]
  13. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bac-teria. Journal of Bacteriology 66:24–26
    [Google Scholar]
  14. Jacobs N. J., Conti S. F. 1965; Effect of hemin on the formation of the cytochrome system of anaerobically grown Staphylococcus epidermidis. Journal of Bacteriology 89:675–679
    [Google Scholar]
  15. Jacobs N. J., Johantges J., Deibel R. H. 1963; Effect of anaerobic growth on nitrate reduction by Staphylococcus epidermidis. Journal of Bacteriology 85:782–787
    [Google Scholar]
  16. Jacobs N. J., Maclosky E. R., Conti S. F. 1967; Effect of oxygen and heme on the development of a microbial respiratory system. Journal of Bacteriology 93:278–285
    [Google Scholar]
  17. Kloos W. E., Schleifer K. H. 1975; Simplified schema for routine identification of human Staphylococcus species. Journal of Clinical Microbiology 1:82–88
    [Google Scholar]
  18. Kocur M., Mortensen N. 1967; Comparison of methods for estimation of anaerobic production of acid from glucose and mannitol in staphylococci and micrococci. Acta pathologica et microbiologica scandinavica 71:141–146
    [Google Scholar]
  19. Moss F. 1956; Adaptation of the cytochromes of Aerobacter aerogenes in response to environmental oxygen tension. Australian Journal of Experimental Biological & Medical Science 34:395–406
    [Google Scholar]
  20. Nishine A., Iwami S., Takahashi M., Yoshida K. 1976; Epidemiologic application of a typing method for Staphylococcus epidermidis strains by the serum-soft agar technic. American Journal of Clinical Pathology 65:711–714
    [Google Scholar]
  21. Oeding P., Digranes A. 1977; Classification of coagulase-negative staphylococci in the diagnostic laboratory. Acta pathologica et microbiologica scandinavica 85:136–142
    [Google Scholar]
  22. Ohtomo T., Yoshida K., San Clemente C. L. 1981; Effect of bile acid derivatives on taurine bio-synthesis and extracellular slime production in en-capsulated Staphylococcus aureus S-7. Infection and Immunity 31:798–807
    [Google Scholar]
  23. Pennock C. A., Huddy R. B. 1967; Phosphatase reaction of coagulase-negative staphylococci and micrococci. Journal of Pathology and Bacteriology 93:685–688
    [Google Scholar]
  24. Schaeffer P. 1952; Recherches sur le métabolisme bact#x00E9;rien des cytochromes et des porphyrines. 1. Disparition partielle des cytochromes par culture anaérobie chez certaines bactéries aérobies facultatives. Biochimica et biophysica acta 9:261–270
    [Google Scholar]
  25. Sivakanesan R., Dawes E. A. 1980; Anaerobic glucose and serine metabolism in Staphylococcus epidermidis. Journal of General Microbiology 118:148–157
    [Google Scholar]
  26. Slater E. C. 1949; A respiratory catalyst required for the reduction of cytochrome c by cytochrome b. Biochemical Journal 45:14–30
    [Google Scholar]
  27. Stockland A. E., Sanclemente C. L. 1968; Lactate dehydrogenase activity in certain strains of Staphylococcus aureus. Journal of Bacteriology 95:74–80
    [Google Scholar]
  28. Takahashi M., Yoshida K., San Clemente C. L. 1977; . Relation of colonial morphologies in soft agar to morphological and biological properties of the K-9 strain of Klebsiella pneumoniae and its variants. Canadian Journal of Microbiology 23:448–451
    [Google Scholar]
  29. Usui Y., Narikawa S., Ichiman Y., Yoshida K. 1979; Isolation of serologically heterologous compact-colony forming active substance from a strain of Staphylococcus epidermidis. Japanese Journal of Bacteriology 34:847–851
    [Google Scholar]
  30. Whittenbury R. 1978; Biochemical characteristics of streptococcus species. In Streptococci pp. 51–69 Skinner F. A., Quesnel L. B. Edited by London: Academic Press;
    [Google Scholar]
  31. Yoshida K. 1973; Compact colony forming activity and the effect of compact-type growth of Staphylococcus aureus strains in serum-soft agar. American Journal of Clinical Pathology 59:412–416
    [Google Scholar]
  32. Yoshida K., Smith M. R., Naito Y. 1971; Compact-type growth of Staphylococcus epidermidis strains in serum-soft agar. Infection and Immunity 4:656–657
    [Google Scholar]
  33. Yoshida K., Smith M. R., Naito Y. 1972; Sero-logical typing of Staphylococcus epidermidis strains by the serum-soft agar technique. Infection and Immunity 5:8–11
    [Google Scholar]
  34. Yoshida K., Nakamura A., Ohtomo T., Iwami S. 1974; Detection of capsular antigen production in unencapsulated strains of Staphylococcus aureus. Infection and Immunity 9:620–623
    [Google Scholar]
  35. Yoshida K., Ohtomo T., Minegishi Y. 1977; Mechanism of compact-colony formation by strains of Staphylococcus aureus in serum-soft agar. Journal of General Microbiology 98:67–75
    [Google Scholar]
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