Summary: The biomass and residual limiting substrate profiles of chemostat grown were typical of carbon- and of nitrogen-limited micro-organisms. Maintenance energy requirements partly accounted for the discrepancy between observed and predicted values of biomass concentration at low dilution rates under glucose limitation. At such low dilution rates the fungal mycelium became differentiated and sporulation occurred. The steady state glucose concentrations in glucose-limited cultures were higher than those predicted by chemostat theory; much closer agreement between observed and predicted values was achieved when a corrected μ term (Pitt & Bull, 1982 a) was used to calculate the residual glucose concentration. The measured growth parameters (μ, μ, , and ) for had values similar to those reported for other species of filamentous fungi. The biomass concentration of fed-batch cultures increased linearly with time but glucose supplied at a rate three times higher than the maintenance requirement was insufficient to prevent sporulation. Mycelial RNA and protein concentrations increased with increasing dilution rate and were 30 and 40% lower under nitrogen limitation than under carbon limitation. DNA concentration was not influenced by dilution rate. The molar ratio of RNA: Mg : K was 8:1:8 and was dilution rate independent. Only two polyamines, spermine and spermidine, were detected in ; they increased in concentration and proportion of the biomass as the dilution rate was raised but there was no evidence of their functional interchange ability with Mg ions as reported for other fungi.


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