1887

Abstract

isolates from ovine virulent footrot and ovine benign footrot and bovine isolates of low virulence for sheep were distinguishable from each other by their proteinase isoenzyme patterns after polyacrylamide gel electrophoresis. Variants of low virulence were not always distinguishable from their virulent parent strains. The molecular weights of the isoenzymes ranged from 70000 to 129000. The relationship of isoenzyme patterns to virulence is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-4-809
1982-04-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/4/mic-128-4-809.html?itemId=/content/journal/micro/10.1099/00221287-128-4-809&mimeType=html&fmt=ahah

References

  1. Beveridge W. I. B. 1941; Foot rot in sheep: a transmissible disease due to infection with Fusiformis nodosus (n.sp.). Studies on its cause, epidemiology and control. Bulletin of the Council of Scientific and Industrial Research of Australia no. 140:1–56
    [Google Scholar]
  2. Depiazzi L. J., Richards A. B. 1979; A degrading proteinase test to distinguish benign and virulent ovine isolates of Bacteroides nodosus. Australian Veterinary Journal 55:25–28
    [Google Scholar]
  3. Egerton J. R., Parsonson I. M. 1969; Benign foot rot. A specific interdigital dermatitis of sheep associated with infection by less proteolytic strains of Fusiformis nodosus. Australian Veterinary Journal 45:345–349
    [Google Scholar]
  4. Every D. 1981a; A new method of sample application for horizontal slab polyacrylamide gel electrophoresis. Analytical Biochemistry 115:7–10
    [Google Scholar]
  5. Every D. 1981b; Quantitative measurement of protease activity in slab polyacrylamide gel electro- phoretograms. Analytical Biochemistry 116:519–523
    [Google Scholar]
  6. Laing E. A., Egerton J. R. 1978; The occurrence, prevalence and transmission of Bacteroides nodosus infection in cattle. Research in Veterinary Science 24:300–304
    [Google Scholar]
  7. Skerman T. M. 1975; Determination of some in vitro growth requirements of Bacteroides nodosus. Journal of General Microbiology 87:107–119
    [Google Scholar]
  8. Skerman T. M., Erasmuson S. K., Every D. 1981; Differentiation of Bacteroides nodosus biotypes and colony variants in relation to their virulence and immunoprotective properties in sheep. Infection and Immunity 32:788–795
    [Google Scholar]
  9. Slater G. G. 1969; Stable pattern formation and determination of molecular size by pore-limit electrophoresis. Analytical Chemistry 41:1039–1041
    [Google Scholar]
  10. Stewart D. J. 1979; The role of elastase in the differentiation of Bacteroides nodosus infections in sheep and cattle. Research in Veterinary Science 27:99–105
    [Google Scholar]
  11. Thomas J. H. 1962; The differential diagnosis of foot rot in sheep. Australian Veterinary Journal 38:159–163
    [Google Scholar]
  12. Wyckoff M., Rodbard D., Chrambach A. 1977; Polyacrylamide gel electrophoresis in sodium dodecyl sulfate-containing buffers using multiphasic buffer systems: properties of the stock, valid Rf-measurement, and optimized procedure. Analytical Biochemistry 78:459–482
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-4-809
Loading
/content/journal/micro/10.1099/00221287-128-4-809
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error