1887

Abstract

A high molecular weight polysaccharide sedimenting at 105 000 was extracted into the water phase of phenol/water extracts of PCC 6912 cells. The main sugars of the heteropolysaccharide were mannose, 6--methyl--mannose, glucose, rhamnose, and glucuronic and galacturonic acids. 6--Methyl--mannose was present partly in terminal linkage and partly 1,3-chain-linked, as revealed by methylation analysis. 3--Methyl-mannose, however, was found in only trace amounts and exclusively occupied terminal positions. The heteropolysaccharide presumably represented a polysaccharide of the cell wall. There was no indication of a lipid moiety. Application of procedures commonly used for lipopolysaccharide extraction from whole cells resulted in very low yields or even indicated complete lack of a typical lipopolysaccharide.

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/content/journal/micro/10.1099/00221287-128-2-273
1982-02-01
2021-07-26
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References

  1. Cardemil L., Wolk C. P. 1979; The polysaccharides from heterocyst and spore envelopes of a blue-green alga. Journal of Biological Chemistry 254:736–741
    [Google Scholar]
  2. Drews G., Meyer H. 1964; Untersuchungen zum chemischen Aufbau der Zellwände von Anacystis nidulans; und Chlorogloea fritschii. . Archiv für Mikrobiologie 48:259–267
    [Google Scholar]
  3. Drews G., Weckesser J. 1981; Function, structure and composition of cell walls and external layers. In The Biology of Cyanobacteria in the Press Edited by Carr N. G., Whitton B. A. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  4. Fichtinger-Schepman A. J., Kamerling J. P., Vliegenthart J. F. G., De Jong E. W., Bosch L., Westbroek P. 1979; Composition of a methylated, acidic polysaccharide associated with coccoliths of Emiliana huxleyi; (Lohmann) Kamptner. Carbohydrate Research 69:181–189
    [Google Scholar]
  5. Fournet B., Leroy Y., Montreuil J., Mayer H. 1974; Analytical and preparative gas-liquid chromatography of methyl-α-d-mannoside monomethyl ethers. Journal of Chromatography 92:185–190
    [Google Scholar]
  6. Galanos C., Lüderitz O., Westphal O. 1969; A new method for the extraction of R-lipopoly- saccharides. European Journal of Biochemistry 9:245–249
    [Google Scholar]
  7. Jansson P.-E., Kenne L., Liedgren H., Lindberg B., Lönngren I. 1979; A practical guide to the methylation analysis of carbohydrates. Chemical Communications, University of Stockholm 8:1–75
    [Google Scholar]
  8. Mikheyskaya L. V., Ovodova R. G., Ovodov Y. S. 1977; Isolation and characterization of lipo- polysaccharides from cell walls of blue-green algae of the genus Phormidium. . Journal of Bacteriology 130:1–3
    [Google Scholar]
  9. Schmidt W., Drews G., Weckesser J., Mayer H. 1980; Lipopolysaccharides in four strains of the unicellular cyanobacterium Synechocystis. . Archives of Microbiology 127:217–222
    [Google Scholar]
  10. Schrader M., Drews G., Golecki J., Weckesser J. 1982; Isolation and characterization of the sheath from the cyanobacterium Chlorogloeopsis; PCC 6912. Journal of General Microbiology 128:267–272
    [Google Scholar]
  11. Tharanathan R. N., Mayer H., Weckesser J. 1978; Location of O-methyl sugars in antigenic (lipo)polysaccharides of photosynthetic bacteria and cyanobacteria. Biochemical Journal 171:403–408
    [Google Scholar]
  12. Van Eykelenburg C. 1978; A glucan from the cell wall of the cyanobacterium Spirulina platensis. . Antonie van Leeuwenhoek 43:321–327
    [Google Scholar]
  13. Wang A. W., Hill A. 1977; Chemical analysis of the phenol-water extractable materials from Anabaena flos-aquae. . Journal of Bacteriology 130:558–560
    [Google Scholar]
  14. Weckesser J., Drews G., Mayer H. 1979; Lipopolysaccharides of photosynthetic prokaryotes. Annual Review of Microbiology 33:215–239
    [Google Scholar]
  15. Westphal O., Lüderitz O., Bister G. 1952; Uber die Extraktion von Bakterien mit Phenol/ Wasser. Zeitschrift für Naturforschung 7b:148–155
    [Google Scholar]
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