1887

Abstract

The determinant(s) of gonococcal resistance to killing by human polymorphonuclear (PMN) phagocytes appear to be present in outer membrane vesicles (OMV) purified from lithium chloride extracts of strain BS4 (agar) by wheat germ agglutinin (WGA) affinity chromatography. OMV neutralized the ability of antisera raised against whole gonococci to drastically reduce the capacity of strain BS4 (agar) to survive within PMN phagocytes. Furthermore, analysis by SDS-PAGE of OMV/WGA precipitates from lithium chloride extracts of strain BS4 (agar) and strain BSSH, which was more susceptible to phagocyte killing than strain BS4 (agar) and yielded OMV with poor antiserum-neutralizing activity, suggested that three proteins were associated with resistance to phagocyte killing.

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/content/journal/micro/10.1099/00221287-128-12-3077
1982-12-01
2021-10-18
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References

  1. Bloch R., Burger M.M. 1974; Purification of wheat germ agglutinin using affinity chromatography on chitin.. Biochemical and Biophysical Research Communications 58:13–19
    [Google Scholar]
  2. Brooks G.F"GOTSCHLICH, Holmes K.K., Sawyer W.D., Young F.E. 1978 Immunobiology of Neisseria gonorrhoeae. Washington D.C.:: American Society for Microbiology.;
    [Google Scholar]
  3. Embo Worksho 1980 Genetics and Immunobiology of Pathogenic Neisseria. Danielsson D., Normark. S. Edited by Proceedings of an EMBO Workshop, Hemavan,; Sweden.:
    [Google Scholar]
  4. Karkhanis T.D., Zeltner J.Y., Jackson J.J., Carlo D.J. 1978; A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of gram-negative bacteria.. Analytical Biochemistry 85:595–601
    [Google Scholar]
  5. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. . Nature; London: 227:680–685
    [Google Scholar]
  6. Lambden P.R., Heckels J.E., James L.T., Watt P.J. 1979; Variations in surface proteins associated with virulence properties in opacity types of Neisseria gonorrhoeae. . Journal of General Microbiology 114:305–312
    [Google Scholar]
  7. Mcbride H.M., Lambden P.R., Heckels J.E., Watt P.J. 1981; The role of outer membrane proteins in the survival of Neisseria gonorrhoeae P9 within guinea-pig subcutaneous chambers.. Journal of General Microbiology 126:63–67
    [Google Scholar]
  8. Parsons N.J., Kwaasi A.A.A., Turner J.A., Veale D.R., Perera V.Y., Penn C.W., Smith H. 1981; Investigation of the determinants of the survival of Neisseria gonorrhoeae within human polymorphonuclear phagocytes.. Journal of General Microbiology 127:103–112
    [Google Scholar]
  9. Penn C.W., Sen D., Veale D.R., Witt K., Parsons N.J., Smith H. 1976; Morphological, biological and antigenic properties of Neisseria gonorrhoeae adapted to growth in guinea pig subcutaneous chambers.. Journal of General Microbiology 97:35–43
    [Google Scholar]
  10. Perera V.Y., Penn C.W., Smith H. 1982; Purification of pili and outer membrane vesicles of Neisseria gonorrhoeae by wheat germ agglutinin affinity chromatography.. Journal of General Microbiology 128:1613–1622
    [Google Scholar]
  11. Veale D.R., Penn C.W., Sutton S., Smith H. 1978; The effect of specific antiserum on the resistance of Neisseria gonorrhoeae to intracellular killing by phagocytes of human blood.. Journal of General Microbiology 106:129–136
    [Google Scholar]
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