1887

Abstract

Intact cells of the methane-oxidizing organism (Bath) assimilated CO for several hours in the absence of methane, provided that an alternative energy source such as hydrogen and/or formate was available. Despite the presence of ribulose bisphosphate carboxylase and a ribulose monophosphate pathway in this organism, autotrophic growth in the presence of a suitable energy source could not be demonstrated. Radiolabelling studies suggested that the CO was incorporated via C carboxylation and ribulose bisphosphate carboxylase in the presence of both methane and hydrogen plus formate. However, in the presence of methane the CO was further metabolized into sugar phosphates, whereas in the absence of methane, but with hydrogen plus formate as an energy source, the sugar phosphates were not labelled to any significant extent. Of the methane-oxidizing bacteria tested, ribulose bisphosphate carboxylase was found only in strains (Bath and Foster & Davis). The ribulose monophosphate pathway in (Bath) probably uses the keto-deoxy-6-phosphogluconate route for the cleavage of fructose 6-phosphate into two C molecules rather than via phosphofructokinase and fructose bisphosphate aldolase which have low activities in this organism. The function of the ribulose bisphosphate carboxylase may be to provide an alternative cleavage pathway for the synthesis of 3-phosphoglycerate during growth on methane.

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/content/journal/micro/10.1099/00221287-128-12-2927
1982-12-01
2024-05-07
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References

  1. Cooper R. A., Kornberg H. L. 1969; Phosphoenolpyruvate synthetase. Methods in Enzymology 13:309–314
    [Google Scholar]
  2. Dalton H., Whittenbury R. 1976; The acetylene reduction technique as an assay for the nitrogenase activity in the methane oxidizing bacterium Methylococcus capsulatus strain Bath. Archives of Microbiology 109:147–151
    [Google Scholar]
  3. Kuby S. A., Noltmann E. A. 1966; Glucose 6-phosphate dehydrogenase (crystalline) from brewer’s yeast. Methods in Enzymology 9:116–125
    [Google Scholar]
  4. Ling K. H., Paetkau V., Marcus F., Lardy H. A. 1966; Phosphofructokinase. Methods in Enzymology 9:425–429
    [Google Scholar]
  5. Linton J. D., Cripps R. E. 1978; The occurrence and identification of intracellular polyglucose storage granules in Methylococcus NCIB 11083 grown in chemostat cultures on methane. Archives of Microbiology 117:41–48
    [Google Scholar]
  6. Malik K. A., Schlegel H. G. 1981; Chemolitho-autotrophic growth of bacteria able to grow under N2-fixing conditions. FEMS Microbiology Letters 11:63–67
    [Google Scholar]
  7. Noltmann E. A. 1966; Phosphoglucose isomerase. Methods in Enzymology 9:557–565
    [Google Scholar]
  8. Pontremoli S., Grazi E. 1966; 6-Phospho-gluconate dehydrogenase. Methods in Enzymology 9:137–142
    [Google Scholar]
  9. Quayle J. R. 1966; Formate dehydrogenase. Methods in Enzymology 9:360–364
    [Google Scholar]
  10. Quayle J. R. 1979; Microbial assimilation of C1 compounds. Biochemical Society Transactions 8:1–10
    [Google Scholar]
  11. Reed H. L. 1976 A study of certain unusual biochemical and physiological properties of obligate methane utilizing bacteria Ph.D. thesis University of Warwick:
    [Google Scholar]
  12. Rutter W. J., Hunsley J. R. 1966; Fructose diphosphate aldolase. Methods in Enzymology 9:480–486
    [Google Scholar]
  13. Stirling D. I., Dalton H. 1978; Purification and properties of an NAD(P)+-linked formaldehyde dehydrogenase from Methylococcus capsulatus (Bath). Journal of General Microbiology 107:19–29
    [Google Scholar]
  14. Strom T., Ferenci T., Quayle J. R. 1974; The carbon assimilation pathways of Methylococcus capsulatus, Pseudomonas methanica and Methylosinus trichosporium (OB3b) during growth on methane. Biochemical Journal 144:465–476
    [Google Scholar]
  15. Taylor S. C. 1977; Evidence for the presence of ribulose-1,5-bisphosphate carboxylase and phosphoribulokinase in Methylococcus capsulatus (Bath). FEMS Microbiology Letters 2:305–307
    [Google Scholar]
  16. Taylor S. C. 1979 Ribulose 1,5-bisphosphate carboxylase and carbon dioxide fixation in Rhodomicrobium vannielii (RM5) and Methylococcus capsulatus (Bath) Ph.D. thesis University of Warwick:
    [Google Scholar]
  17. Taylor S. C., Dalton H., Dow C. S. 1981; Ribulose-1,5-bisphosphate carboxylase/oxygenase and carbon assimilation in Methylococcus capsulatus(Bath). Journal of General Microbiology 122:89–94
    [Google Scholar]
  18. Whittenbury R., Dalton H. 1981; The methylotrophic bacteria. In The Prokaryotes pp. 894–902 Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Edited by Berlin: Springer-Verlag;
    [Google Scholar]
  19. Whittenbury R., Phillips K. C., Wilkinson J. F. 1970; Enrichment, isolation and some properties of methane-utilizing bacteria. Journal of General Microbiology 61:205–218
    [Google Scholar]
  20. Whittenbury R., Dalton H., Eccleston M., Reed H. L. 1975; The different types of methane oxidizing bacteria and some of their more unusual properties. In Microbial Growth on C1 Compounds: Proceedings of the International Symposium on Microbial Growth on C1-Compounds pp. 1–9 Teriu G. Edited by Tokyo. Osaka: Society of Fermentation Technology;
    [Google Scholar]
  21. Wood W. A. 1971; Assays of enzymes representative of metabolic pathways VI 2-keto-3-deoxy-6-phosphogluconicaldolase. Methods in Microbiology 6A:411–424
    [Google Scholar]
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