1887

Abstract

Plasmids R394a and R394b which cointegrate to form R394 are described. They have molecular masses of 102 ± 4 and 11·0 ± 0·4 MDal, belong to the T and N incompatibility groups and confer resistance to kanamycin and ampicillin, respectively. R394a is self transmissible and mobilizes R394b, which is non-self transmissible. These findings clarify anomalies in the behaviour of R394 and support suggestions based on the properties of variant phages derived from R394.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-11-2791
1982-11-01
2021-05-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/11/mic-128-11-2791.html?itemId=/content/journal/micro/10.1099/00221287-128-11-2791&mimeType=html&fmt=ahah

References

  1. Beck Y., Coetzee J. N. 1980; Mobilization of the Proteus morganii chromosome by R plasmids. Journal of General Microbiology 118:539–542
    [Google Scholar]
  2. Bradley D. E., Coetzee J. N., Bothma T., Hedges R. W. 1981; Phage t: a group T plasmid-dependent bacteriophage. Journal of General Microbiology 126:397–403
    [Google Scholar]
  3. Clowes R. C., Hayes W. 1968 Experiments in Microbial Genetics Oxford and Edinburgh: Blackwell Scientific Publications;
    [Google Scholar]
  4. Coetzee J. N. 1974; High frequency transduction of kanamycin resistance in Proteus mirabilis. Journal of General Microbiology 84:285–296
    [Google Scholar]
  5. Coetzee J. N. 1975a; High frequency transduction of resistance to ampicillin and kanamycin in Proteus mirabilis. Journal of General Microbiology 87:173–176
    [Google Scholar]
  6. Coetzee J. N. 1975b; Specialized transduction of kanamycin resistance in a Providence strain. Journal of General Microbiology 88:307–316
    [Google Scholar]
  7. Coetzee J. N. 1979; Genetic circularity of the Proteus mirabilis linkage map. Journal of General Microbiology 110:171–176
    [Google Scholar]
  8. Coetzee J. N., Krizsanovich-Williams K. 1976; Transduction of leucineauxotrophs of Proteus mirabilis to prototrophy or antibiotic resistance by P. mirabilis high frequency transducing bacteriophages. Journal of General Microbiology 92:369–374
    [Google Scholar]
  9. Coetzee W. F., Pretorius G. H. J. 1979; Factors which influence the electron microscopic appearance of DNA when benzyldimethylalkylammonium chloride is used. Journal of Ultrastructure Research 67:33–39
    [Google Scholar]
  10. Coetzee J. N., Datta N., Hedges R. W. 1972; R factors from Proteus rettgeri. Journal of General Microbiology 72:543–552
    [Google Scholar]
  11. Coetzee J. N., Datta N., Hedges R. W., Appelbaum P. C. 1973; Transduction of R factors in Proteus mirabilis and P. rettgeri. Journal of General Microbiology 76:355–368
    [Google Scholar]
  12. Cohen S. N., Chang A. C. Y., Hsu L. 1972; Non chromosomal antibiotic resistance in bacteria: Genetic transformation of Escherichi coli by R-factor DNA. Proceedings of the National Academy of Sciences of the United States of America 69:2110–2114
    [Google Scholar]
  13. Datta N., Hedges R. W. 1971; Compatibility groups among fi R factors. Nature; London: 234222–223
    [Google Scholar]
  14. Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. 1971; Properties of an R factor from Pseudomonas aeruginosa. Journal of Bacteriology 108:1244–1249
    [Google Scholar]
  15. Gill R., Heffron F., Dougan G., Falkow S. 1978; Analysis of sequences transposed by complementation of two classes of transposition deficient mutants of Tn3. Journal of Bacteriology 136:742–756
    [Google Scholar]
  16. Hedges R. W., Datta N., Kontomichalou P., Smith J. T. 1974; Molecular specificities of R factor-determined beta-lactamases: Correlation with plasmid compatibility. Journal of Bacteriology 117:56–62
    [Google Scholar]
  17. Jacob A. E., Shapiro J. A., Yamamoto L., Smith D. I., Cohen S. N., Berg D. 1977; Plasmids studied in Escherichia coli and other enteric bacteria. In DNA Insertion Elements, Plasmids, and Episomes pp. 607–638 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Matsumoto H., Kamio Y., Kobayashi R., Terawaki Y. 1978; R plasmid Rtsl-mediated production of extracellular deoxyribonuclease in Escherichia coli. Journal of Bacteriology 133:387–389
    [Google Scholar]
  19. Matthew M., Hedges R. W. 1976; Analytical isoelectric focusing of R factor-determined β-lacta- mases: correlation with plasmid compatibility. Journal of Bacteriology 125:713–718
    [Google Scholar]
  20. Prakash R. K., Schilperoort R. A., Nuti M. P. 1981; Large plasmids of fast growing Rhizobia: homology studies and location of structural nitrogen fixation (nif) genes. Journal of Bacteriology 145:1129–1136
    [Google Scholar]
  21. Pretorius G. H. J., Coetzee W. F. 1980; Proteus mirabilis phages 5006M, 5006M HFT k and 5006M HFT ak: physical comparison of genome characteristics. Journal of General Virology 49:33–39
    [Google Scholar]
  22. Stanisich V. A. 1974; The properties and host range of male-specific bacteriophages of Pseudomonas aeruginosa. Journal of General Microbiology 84:332–342
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-11-2791
Loading
/content/journal/micro/10.1099/00221287-128-11-2791
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error