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Plasmids R394a and R394b which cointegrate to form R394 are described. They have molecular masses of 102 ± 4 and 11·0 ± 0·4 MDal, belong to the T and N incompatibility groups and confer resistance to kanamycin and ampicillin, respectively. R394a is self transmissible and mobilizes R394b, which is non-self transmissible. These findings clarify anomalies in the behaviour of R394 and support suggestions based on the properties of variant phages derived from R394.
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