1887

Abstract

A method has been devised for isolating yeast ascospores and asci free from vegetative cell contamination. By careful manipulation of the size and surface properties of the spheroplasted vegetative cells and asci, a pure preparation of ascospores could be recovered by sucrose gradient centrifugation.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-11-2725
1982-11-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/11/mic-128-11-2725.html?itemId=/content/journal/micro/10.1099/00221287-128-11-2725&mimeType=html&fmt=ahah

References

  1. Betz H., Weiser U. 1976; Protein degradation and proteinases during yeast sporulation. European Journal of Biochemistry 62:65–76
    [Google Scholar]
  2. Dawes I. W., Hardie I. D. 1974; Selective killing of vegetative cells in sporulated yeast culture by exposure of diethyl ether. Molecular and General Genetics 131:281–289
    [Google Scholar]
  3. Dawes I. W., Wright J. F., Vezinhet F., Ajam N. 1980; Separation on urografin gradients of subpopulations from sporulating Saccharomyces cerevi- siae cultures. Journal of General Microbiology 119:165–171
    [Google Scholar]
  4. Esposito M. S., Esposito R. E. 1974; Genes controlling meiosis and spore formation in yeast. Genetics 78:215–225
    [Google Scholar]
  5. Klein H. L., Byers B. 1978; Stable denaturation of chromosomal DNA from Saccharomyces cerevisiae during meiosis. Journal of Bacteriology 134:629–635
    [Google Scholar]
  6. Nishi K., Shimoda C., Hayashibe M. 1978; Germination and outgrowth of Schizosaccharomyces pombe ascospores isolated by urografin density-gradient centrifugation. Canadian Journal of Microbiology 24:893–897
    [Google Scholar]
  7. Padilla G. M., Carter B. L. A., Mitchison J. M. 1975; Germination of Schizosaccharomyces pombe spores separated by zonal centrifugation. Experimental Cell Research 93:325–330
    [Google Scholar]
  8. Piñon R., Salts Y., Simchen G. 1974; Nuclear and mitochondrial DNA synthesis during yeast sporulation. Experimental Cell Research 83:231–238
    [Google Scholar]
  9. Resnick M. A., Tippetts R. D., Mortimer R. K. 1967; Separation of spores from diploid cells of yeast by stable-flow free-boundary electrophoresis. Science 158:803–804
    [Google Scholar]
  10. Roth R., Fogel S. 1971; A selective system for yeast mutants deficient in meiotic recombination. Molecular and General Genetics 112:295–305
    [Google Scholar]
  11. Rousseau P., Halvorson H. O. 1969; Preparation and storage of single spores of Saccharomyces cerevi- siae. Journal of Bacteriology 100:1426–1427
    [Google Scholar]
  12. Simchen G., Piñon R., Salts Y. 1972; Sporula- tion in Saccharomyces cerevisiae. Premeiotic DNA synthesis, readiness and commitment. Experimental Cell Research 75:207–218
    [Google Scholar]
  13. Shilo V., Simchen G., Shilo B. 1978; Initiation of meiosis in cell cycle initiation mutants of Saccharomyces cerevisiae. Experimental Cell Research 112:241–248
    [Google Scholar]
  14. Tingle M. A., Kuenzi M. T., Halvorson H. O. 1974; Germination of yeast spores lacking mitochondrial deoxyribonucleic acid. Journal of Bacteriology 117:89–93
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-11-2725
Loading
/content/journal/micro/10.1099/00221287-128-11-2725
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error