1887

Abstract

The turimycin-producing strain JA6599/NG60-93 (TurAmy) and strain CCl, which is unable to produce antibiotic and aerial mycelium (TurAmy), were compared with respect to their mycelial enzyme activities and cellular lipid composition. Changed activities of six enzymes of intermediary metabolism during submerged growth of strain CCl on chemically defined medium attest to alterations of the life cycle. In addition, strain CCl contained decreased amounts of 12-methyltetradecanoic acid in relation to 14-methylpentadecanoic acid (isopalmitic acid) and displayed a quantitatively altered phospholipid composition.

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1982-11-01
2024-12-10
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References

  1. Arx E., Von. 1963; Eine multidimensionale Technik zur chromatographischen Identifizierung von Aminosauren. Journal of Chromatography 12:329–341
    [Google Scholar]
  2. Batrakov S. G., Bergelson L. D. 1978; Lipids of streptomycetes.Structural investigation and biological interrelation. Chemistry and Physics of Lipids 21:1–29
    [Google Scholar]
  3. Bergmeyer H. U. 1970; Malat-Dehydrogenase. UV-Test. In Methoden der EnzymatischenAnalyse pp. 446–447 Bergmeyer H. U. Edited by Berlin: Akademie-Verlag;
    [Google Scholar]
  4. Bernt E., Bergmeyer H. U. 1970; Isocitrat-Dehydrogenase. UV-Test. In Methoden der EnzymatischenAnalyse pp. 587–590 Bergmeyer H. U. Edited by Berlin: Akademie-Verlag;
    [Google Scholar]
  5. Chater K. F. 1979; Some recent developments in streptomyces genetics. In Genetics of Industrial Microorganisms pp. 123–133 Sebek O. K., Laskin A. I. Edited by Washington, D.C: American Society for Microbiology;
    [Google Scholar]
  6. Ensign J. C. 1978; Formation, properties and germination of actinomycetes spores. Annual Review of Microbiology 32:185–219
    [Google Scholar]
  7. Hopwood D. A. 1978; Extrachromosomally determined antibiotic production. Annual Review of Microbiology 32:373–392
    [Google Scholar]
  8. Kalakoutski L. V., Agre N. S. 1977; Comparative aspects of development and differentiation in actinomycetes. Bacteriological Reviews 40:469–524
    [Google Scholar]
  9. Kates M. 1972; Techniques in lipidology. In Laboratory Techniques in Molecular Biology and Biochemistry pp. 275–600 Work T. S., Work E. Edited by Amsterdam: North Holland Publishing Co;
    [Google Scholar]
  10. Kohlhaw G., Drägert W., Holzer H. 1965; Parallel-Repression der Synthese von Glutamin- Synthetase und DPN-abhängiger Glutamat-Dehydrogenase in Hefe. Biochemische Zeitschrift 341:224–238
    [Google Scholar]
  11. Konova I. V., Kasimbekova C. K., Mitjushina L. L., Batrakov S. G., Bechterova M. N. 1978; Influence of phosphate on composition of polar lipids from Actinomyces olivaceus. Izvestiya Akademii Nauk SSSR, Seriya biologitcheskaya 2:285–291
    [Google Scholar]
  12. Löhr G. W., Waller H. D. 1970; Glucose-6- phosphat-Dehydrogenase. In Methoden der EnzymatischenAnalyse pp. 599–606 Bergmeyer H. U. Edited by Berlin: Akademie-Verlag;
    [Google Scholar]
  13. Machtinger N. A., Fox C. F. 1973; Biochemistry of bacterial membranes. Annual Review of Biochemistry 42:575–600
    [Google Scholar]
  14. Murray W. C., Magee W. L. 1972; Phospholipid metabolism. Annual Review of Biochemistry 41:121–160
    [Google Scholar]
  15. Okanishi M. 1979; Plasmids and antibiotic synthesis in streptomycetes. In Genetics of Industrial Microorganisms pp. 134–140 Sebek O. K., Laskin A. I. Edited by Washington, D.C: American Society for Microbiology;
    [Google Scholar]
  16. Pronina M. I., Efimova T. P., Zhokova R. A., Motovilova T. G., Pechatnikova I. S., Teresin I. M. 1980; Morphological and biochemical characteristics of mutants of Streptomyces hygroscopicus under the action of acridine orange and mitomycin C. Mikrobiologiya 49:302–307
    [Google Scholar]
  17. Roth M., Noack D. 1982; Genetic stability of differentiated functions in Streptomyces hygroscopicus in relation to conditions of continuous culture. Journal of General Microbiology 128:107–114
    [Google Scholar]
  18. Roth M., Noack D., Reinhardt G. 1982; Properties of non-differentiating derivatives of Streptomyces hygroscopicus. Journal of General Microbiology 128:2687–2691
    [Google Scholar]
  19. Schaffer P., Guespin-Machel J., Cami B., Rougard J. F., Mazliak P. 1972; Membrane changes in some pleiotropic sporulation mutants of Bacillus subtilis. In Spore Research 1971 pp. 333–334 Barker A. N., Gould G. W., Wolf J. L. Edited by London: Academic Press;
    [Google Scholar]
  20. Schmidt E. 1970; Glutamat-Dehydrogenase. UV- Test. In Methoden der EnzymatischenAnalyse pp. 609–613 Bergmeyer H. U. Edited by Berlin: Akademie-Verlag;
    [Google Scholar]
  21. Wlame J. M., Pierard A., Ramos F. 1962; L-Alanine dehydrogenase from Bacillus subtilis. Methods in Enzymology 5:673–677
    [Google Scholar]
  22. Willecke K., Pardee A. B. 1971; Fatty acid- requiring mutant of Bacillus subtilis defective in branched chain α-ketoacid dehydrogenase. Journal of Biological Chemistry 246:5264–5272
    [Google Scholar]
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