1887

Abstract

Representatives of groups of mycoplasmas which have antigens in common with (as demonstrated by different serological tests) were compared by nucleic acid hybridization. Determinations of DNA homology were performed by filter hybridization as well as hybridization in solution; no differences were revealed between the two methods. Genetic relatedness was not demonstrated between and strain F38. The antigenic similarities between strain F38 and the type strain of (HRC292) may be due to common epitopes. DNA from strain F38 hybridized with DNA from (California kid) to 80%, but only to about 40% with the two subspecies, a result which can justify the classification of the F38 group as a variant of . The representative strain of bovine serogroup 7 of Leach was equally distant from F38, and the two subspecies of (approximately 60% hybridization).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-11-2523
1982-11-01
2024-10-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/11/mic-128-11-2523.html?itemId=/content/journal/micro/10.1099/00221287-128-11-2523&mimeType=html&fmt=ahah

References

  1. Askaa G., Christiansen C., Ernø H. 1973; Bovine mycoplasmas: genome size and base composition of DNA. Journal of General Microbiology 75:283–286
    [Google Scholar]
  2. Askaa G., Ernø H., Ojo M. O. 1978; Bovine mycoplasmas: classification of groups related to Mycoplasma mycoides. Acta veterinaria scandinavica 19:166–178
    [Google Scholar]
  3. Aulakh G. S., Gallo R. C. 1977; Rauscher-leukemia-virus-related sequences in human DNA: presence in some tissues of some patients with hematopoietic neoplasias and absence in DNA from other tissues. Proceedings of the National Academy of Sciences of the United States of America 74:353–357
    [Google Scholar]
  4. Britten R. J., Graham D. E., Neufeld B. D. 1974; Analysis of repeating DNA sequences by reassociation. Methods in Enzymology 29:363–418
    [Google Scholar]
  5. Christiansen C., Black F. T., Freundt E. A. 1981; Hybridization experiments with deoxyribonucleic acid from Ureaplasma urealyticumserovars I to VIII. International Journal of Systematic Bacteriology 31:259–262
    [Google Scholar]
  6. Cottew G. S., Yeats F. R. 1978; Subdivision of Mycoplasma mycoides subsp.mycoides from cattle and goats into two types. Australian Veterinary Journal 54:293–296
    [Google Scholar]
  7. Denhardt D. T. 1966; A membrane-filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23:641–646
    [Google Scholar]
  8. Ernø H., Salih M. M. 1980; The growth precipitation test as a diagnostic method for differentiation ofMycoplasma and Acholeplasma species. Acta veterinaria scandinavica 21:469–481
    [Google Scholar]
  9. Ernø H., Leach R. H., Macowan K. J. 1979; Further characterization of Mycoplasma strain F38. Tropical Animal Health and Production 11:84
    [Google Scholar]
  10. Johnson J. L. 1973; Use of nucleic-acid homologies in the taxonomy of anaerobic bacteria. International Journal of Systematic Bacteriology 23:308–315
    [Google Scholar]
  11. Kaliner G., Macowan K. J. 1976; The pathology of experimental and natural contagious caprinepleuropneumonia in Kenya. Zentralblatt für Veterinär Medizin 23:652–661
    [Google Scholar]
  12. Macowan K. J., Minette J. E. 1976; A Mycoplasma from acute contagious caprinepleuropneumonia in Kenya. Tropical Animal Health and Production 8:91–95
    [Google Scholar]
  13. Macowan K. J., Minette J. E. 1977; Contact transmission of experimental contagious caprinepleuropneumonia (CCPP). Tropical Animal Health Production 9:185–188
    [Google Scholar]
  14. Macowan K. J., Minette J. E. 1978; The effect of high passage Mycoplasma strain F38 on the course of contagious caprinepleuropneumonia (CCPP). Tropical Animal Health Production 10:31–35
    [Google Scholar]
  15. Rigby P. W., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  16. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. Journal of Molecular Biology 4:430–443
    [Google Scholar]
/content/journal/micro/10.1099/00221287-128-11-2523
Loading
/content/journal/micro/10.1099/00221287-128-11-2523
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error