1887

Abstract

Growing cultures of hydroxylate progesterone at the 6 and 11positions. The properties of the 6-hydroxylase activity were studied in extracts of this organism using 11 hydroxyprogesterone as substrate. Maximum 6-hydroxylase activity was obtained at pH 6·5. EDTA and thiophenol inhibited hydroxylation, while NADPH, 2-oxoglutarate and pyruvate stimulated it. Cobalt and cadmium ions inhibited the 6-hydroxylase activity. The feasibility of introducing a hydroxyl group into the 6-position of various steroid compounds was tested: the 3-Δ configuration appeared to exert a directional effect for 6-hydroxylation, while an 11-hydroxyl group exerted some kind of steric hindrance against 6-hydroxylation.

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/content/journal/micro/10.1099/00221287-128-11-2511
1982-11-01
2022-01-20
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