1887

Microbiology Society logo

Volume 128, Issue 10

The Plasmid-coded Metabolism of Naphthalene and 2-Methylnaphthalene in Strains: Phenotypic Changes Correlated with Structural Modification of the Plasmid pWW60-1 Free

Abstract

sp. NCIB 9816 contains two plasmids: pWW60, an IncP9 plasmid of 87 kb encoding genes for the catabolism of naphthalene, and pWW61, a cryptic plasmid of about 65 kb. The ability to degrade naphthalene was transferred at low frequency by conjugation from strain NCIB 9816 into a plasmid-free strain of , PaW340. A transconjugant, PaW701, containing the naphthalene plasmid pWW60-1, metabolized naphthalene and salicylate via the pathway. 2-Methylnaphthalene was not a growth substrate but was partly metabolized with accumulation of a brown compound in the medium ( = 440 nm). Spontaneous mutants of PaW701 with the ability to grow on 2-methylnaphthalene arose at a frequency of about 10. These fell into two groups. Group A mutants had no detectable salicylate hydroxylase activity and accumulated salicylate from naphthalene in culture supernatants: they appeared to grow on the pyruvate released from oxidation of the first ring of both substrates. Their plasmids all contained a 16·7 kb insert in different sites within a small, limited region of the plasmid. Group B mutants used a pathway for catabolism of naphthalene and 2-methylnaphthalene. Their plasmids had undergone a small deletion of from 1·2 to 1·6 kb in a region of the plasmid close to the sites of the insertions in the group A mutants.

  • Received:
  • Revised:
  • Published Online:
© Society for General Microbiology 1982
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-10-2281
1982-10-01
2024-10-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/10/mic-128-10-2281.html?itemId=/content/journal/micro/10.1099/00221287-128-10-2281&mimeType=html&fmt=ahah

References

  1. Barnsley E. A. 1976; Role and regulation of the ortho and meta pathways of catechol metabolism in pseudomonads metabolising naphthalene and salicylate. Journal of Bacteriology 125:404–408
     [Google Scholar]
  2. Bayley S. A., Duggleby C. J., Worsey M. J., Williams P. A., Hardy K. G., Broda P. 1977; Two modes of loss of the TOL function from Pseudomonas putida mt-2. Molecular and General Genetics 154:203–204
     [Google Scholar]
  3. Bayly R. C., Dagley S. 1969; Oxoenoic acids as metabolites in the bacterial degradation of catechols. Biochemical Journal 111:303–307
     [Google Scholar]
  4. Boronin A. M., Kochetov V. V., Starovoitov 1. 1., Skryabin G. K. 1977; Plasmids pBS2 and pBS3, controlling the oxidation of naphthalene in bacteria of the genus Pseudomonas. . Doklady Akademii nauk SSSR 237:1205–1208
     [Google Scholar]
  5. Boronin A. M., Kochetkov V. V., Skryabin G. K. 1980; Incompatibility groups of naphthalene degradative plasmids in Pseudomonas. . FEMS Microbiology Letters 7:249–252
     [Google Scholar]
  6. Catterall F. A., Sala-Trepat J. M., Williams P. A. 1971; The coexistence of two pathways for the metabolism of 2-hydroxymuconic semialdehyde in a naphthalene-grown pseudomonad. Biochemical and Biophysical Research Communications 43:463–469
     [Google Scholar]
  7. Davies J. I., Evans W. C. 1964; Oxidative metabolism of naphthalene by soil pseudomonads. Biochemical Journal 91:251–261
     [Google Scholar]
  8. Downing R. G., Broda P. 1980; A cleavage map of the TOL plasmid of Pseudomonas putida mt-2. Molecular and General Genetics 117:189–191
     [Google Scholar]
  9. Dunn N. W., Gunsalus I. C. 1973; Transmissible plasmid coding early enzymes of naphthalene oxidation in Pseudomonas putida. . Journal of Bacteriology 114:974–979
     [Google Scholar]
  10. Feist C. F., Hegeman G. D. 1969; Phenol and benzoate metabolism by Pseudomonas putida: regu-lation of tangential pathways. Journal of Bacteriology 100:869–877
     [Google Scholar]
  11. Hayaishi O., Katagiri N., Rothberg A. 1957; Studies on oxygenases: pyrocatechase. Journal of Biological Chemistry 229:905–920
     [Google Scholar]
  12. Jeenes D. J., Williams P. A. 1982; Excision and integration of degradative pathway genes from TOL plasmid pWWO. Journal of Bacteriology 150:188–194
     [Google Scholar]
  13. Jeenes D. J., Reineke W., Knackmuss H-J., Williams P. A. 1982; The TOL plasmid pWWO in constructed halobenzoate degrading Pseudomonas strains: enzyme regulation and DNA structure. Journal of Bacteriology 150:180–187
     [Google Scholar]
  14. Murray K., Duggleby C. J., Sala-Trepat J. M., Williams P. A. 1972; The metabolism of benzoate and methyl benzoates via the meta cleavage pathway by Pseudomonas arvilla mt-2. European Journal of Biochemistry 28:301–310
     [Google Scholar]
  15. Ornston L. N. 1966; The conversion of catechol and protocatechuate to β-ketoadipate by Pseudomonas putida. III. Enzymes of the catechol pathway. Journal of Biological Chemistry 241:3795–3799
     [Google Scholar]
  16. Pickup R., Williams P. A. 1982; Spontaneous deletions in the TOL plasmid pWW20 which give rise to the B3 regulatory mutants. Journal of General Microbiology 128:1385–1390
     [Google Scholar]
  17. Rogoff M. H., Wender I. 1959; Methylnaphtha- lene oxidations by pseudomonads. Journal of Bacteriology 77:783–788
     [Google Scholar]
  18. Sala-Trepat J. M., Murray K., Williams P. A. 1972; The metabolic divergence in the meta cleavage of catechols by Pseudomonas putida NCIB 10015. Physiological significance and evolutionary implications. European Journal of Biochemistry 28:347–356
     [Google Scholar]
  19. Trinder P. 1954; Rapid determination of salicylate in biological fluids. Biochemical Journal 51:301–303
     [Google Scholar]
  20. Wheatcroft R., Williams P. A. 1981; Rapid methods for the study of both stable and unstable plasmids in Pseudomonas. . Journal of General Microbiology 124:433–437
     [Google Scholar]
  21. Williams P. A., Murray K. 1974; Metabolism of benzoate and the methylbenzoates by Pseudomonas putida {arvilla) mt-2: evidence for the existence of a TOL plasmid. Journal of Bacteriology 120:416–423
     [Google Scholar]
  22. Williams P. A., Catterall F. A., Murray K. 1975; Metabolism of naphthalene, salicylate and benzoate by Pseudomonas PG: regulation of tangential pathways. Journal of Bacteriology 124:679–685
     [Google Scholar]
  23. Yamamoto S., Katagiri H., Maeno H., Hayaishi O. 1965; Salicylate hydroxylase, a monooxygenase requiring flavin adenine dinucleotide. I. Purification and general properties. Journal of Biological Chemistry 240:3408–3413
     [Google Scholar]
/content/journal/micro/10.1099/00221287-128-10-2281
Loading
The Plasmid-coded Metabolism of Naphthalene and 2-Methylnaphthalene in Pseudomonas Strains: Phenotypic Changes Correlated with Structural Modification of the Plasmid pWW60-1
Microbiology 128, 2281 (1982); https://doi.org/10.1099/00221287-128-10-2281
/content/journal/micro/10.1099/00221287-128-10-2281
/content/journal/micro/10.1099/00221287-128-10-2281
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error