1887

Abstract

The effect of dissolved oxygen concentration on the metabolism of glucose in was studied with chemostat cultures using both single-step and gradual transitions from either ammonium or glucose limitation to oxygen limitation and studying transient and steady states. The pathway of glucose metabolism was regulated by the availability of oxygen. The organism responded to oxygen limitation by adjusting its metabolism of glucose from the extracellular direct oxidative pathway, which produces gluconate and 2-oxogluconate, to the intracellular phosphorylative route. This change was a consequence of decreased activities of glucose dehydrogenase and gluconate dehydrogenase and of the transport systems for gluconate and 2-oxogluconate, and an increased activity of glucose transport, while relatively high activities of hexokinase and glucose-6-phosphate dehydrogenase were maintained. Citrate synthase, isocitrate dehydrogenase and malate dehydrogenase activities responded to changes in dissolved oxygen concentration rather than to changes in the glucose or ammonium concentrations. The effect of oxygen limitation on the oxo-acid dehydrogenases and aconitase was probably due, wholly or in part, to repression by glucose consequent upon the increase in residual glucose concentration. Succinate dehydrogenase was repressed by an increase in ammonium concentration under an oxygen limitation.

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1982-01-01
2021-07-30
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References

  1. Carter I. S., Dawes E. A. 1979; Effect of oxygen concentration and growth rate on glucose metabolism, poly-β-hydroxybutyrate biosynthesis and respiration of Azotobacter beijerinckii. Journal of General Microbiology 110:393–400
    [Google Scholar]
  2. Chaney A. L., Marbach E. P. 1962; Modified reagents for determination of urea and ammonia. Clinical Chemistry 8:130–132
    [Google Scholar]
  3. Dalton H., Postgate J. R. 1969; Effect of oxygen on growth of Azotobacter chroococcum in batch and continuous culture. Journal of General Microbiology 54:463–473
    [Google Scholar]
  4. Drozd J., Postgate J. R. 1970; Effects of oxygen on acetylene reduction, cytochrome content and respiratory activity of Azotobacter chroococcum. . Journal of General Microbiology 63:63–73
    [Google Scholar]
  5. Gornall A. G., Bardawill C. J., David M. M. 1949; Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry 177:751–766
    [Google Scholar]
  6. Haaker H., Veeger C. 1976; Regulation of respiration and nitrogen fixation in different types of Azotobacter vinelandii. . European Journal of Biochemistry 63:499–507
    [Google Scholar]
  7. Harrison D. E. F. 1976; The regulation of respiration rate in growing bacteria. Advances in Microbial Physiology 14:243–313
    [Google Scholar]
  8. Hunt J. C., Phibbs P. V. Jr 1977 Abstracts of the Annual Meeting of the American Society for Microbiology abstract K5: p. 187
    [Google Scholar]
  9. Jackson F. A., Dawes E. A. 1976; Regulation of the tricarboxylic acid cycle and poly-β-hydroxybutyrate metabolism in Azotobacter beijerinckii grown under nitrogen or oxygen limitation. Journal of General Microbiology 97:303–312
    [Google Scholar]
  10. Jones C. W. 1979; Energy metabolism in aerobes. In Microbial Biochemistry. International Review of Biochemistry 21 pp. 49–84 Quayle J. R. Edited by Baltimore: University Park Press.;
    [Google Scholar]
  11. Lanning M. C., Cohen S. S. 1951; The detection and estimation of 2-ketohexonic acids. Journal of Biological Chemistry 189:109–114
    [Google Scholar]
  12. Midgley M., Dawes E. A. 1973; The regulation of transport of glucose and methyl α-glucoside in Pseudomonas aeruginosa. . Biochemical Journal 132:141–154
    [Google Scholar]
  13. Ng F.M.-W., Dawes E. A. 1973; Chemostat studies on the regulation of glucose metabolism in Pseudomonas aeruginosa by citrate. Biochemical Journal 132:129–140
    [Google Scholar]
  14. Parker C. A. 1954; Effect of oxygen on nitrogen fixation by Azotobacter. Nature; London: 173780–781
    [Google Scholar]
  15. Phillips D. A., Johnson M. J. 1961; Aeration in fermentations. Journal of Biochemical and Microbiological Technology and Engineering 3:277–309
    [Google Scholar]
  16. Racker E. 1950; Spectrophotometric measurements of the enzymatic formation of fumaric and cis- aconitic acids. Biochimica et biophysica acta 4:211–214
    [Google Scholar]
  17. Roberts B. K., Midgley M., Dawes E. A. 1973; The metabolism of 2-oxogluconate by Pseudomonas aeruginosa. . Journal of General Microbiology 78:319–329
    [Google Scholar]
  18. Senior P. J., Dawes E. A. 1973; The regulation of poly-β-hydroxybutyrate metabolism in Azotobacter beijerinckii. . Biochemical Journal 134:225–228
    [Google Scholar]
  19. Senior P. J., Beech G. A., Ritchie G. A. F., Dawes E. A. 1972; The role of oxygen limitation in the formation of poly-β-hydroxybutyrate during batch and continuous culture of Azotobacter beijerinckii. . Biochemical Journal 128:1193–1201
    [Google Scholar]
  20. Stephenson M. P., Jackson F. A., Dawes E. A. 1978; Further observations on carbohydrate metabolism and its regulation in Azotobacter beijerinckii. . Journal of General Microbiology 109:89–96
    [Google Scholar]
  21. Stouthamer A. H. 1978; Energy-yielding pathways. In The Bacteria 6 pp. 389–462 Ornston L. N., Sokatch J. R. Edited by New York: Academic Press.;
    [Google Scholar]
  22. Veeger C., Der Vartanian D. V., Zeylemaker W. P. 1969; Succinate dehydrogenase. Methods in Enzymology 13:81–90
    [Google Scholar]
  23. Von Tigerström M., Campbell J. J. R. 1966; The accumulation of α-ketoglutarate by suspensions of Pseudomonas aeruginosa. . Canadian Journal of Microbiology 12:1005–1013
    [Google Scholar]
  24. Ward A. C., Rowley B. I., Dawes E. A. 1977; Effect of oxygen and nitrogen limitation on poly- β-hydroxybutyrate biosynthesis in ammonium-grown Azotobacter beijerinckii. . Journal of General Microbiology 102:61–68
    [Google Scholar]
  25. Weitzman P. D. J., Dunmore P. 1969; Regulation of citrate synthase activity by α-ketoglutarate. Metabolic and taxonomic significance. FEBS Letters 3:265–267
    [Google Scholar]
  26. Whiting P. H., Midgley M., Dawes E. A. 1976a; The regulation of transport of glucose, gluconate and 2-oxogluconate and of glucose catabolism in Pseudomonas aeruginosa. . Biochemical Journal 154:659–668
    [Google Scholar]
  27. Whiting P. H., Midgley M., Dawes E. A. 1976b; The role of glucose limitation in the regulation of the transport of glucose, gluconate and 2-oxogluconate, and of glucose metabolism in Pseudomonas aeruginosa. . Journal of General Microbiology 92:304–310
    [Google Scholar]
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