1887

Abstract

Mutants of that developed huge aggregation streams in expanding clones were investigated using optical and biochemical techniques. Representatives of the six complementation groups previously identified () were found to be similar to the parental wild-type strain XP55 in both the extent and timing of their ability to initiate and relay chemotactic signals and in the formation of cyclic AMP receptors and phosphodiesterases. The mutants differed from the wild-type in producing an abnormal chemotactic (movement) response visible using both dark-field optics with synchronously aggregating amoebae on solid substrata and light scattering techniques with oxygenated cell suspensions. Mutants of complementation group showed chemotactic movement responses lasting up to 520 s, rather than 100 s as seen in the parental and other strains. Measurements of cyclic GMP formed intracellularly in response to chemotactic pulses of cyclic AMP in mutants showed that abnormally high concentrations of this nucleotide were formed within 10 s and were not rapidly degraded. A causal correlation between defective cyclic GMP metabolism and the altered chemotactic response is suggested, and a model is proposed that accounts for the formation of huge aggregation streams in clones of these mutants.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-127-2-339
1981-12-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/127/2/mic-127-2-339.html?itemId=/content/journal/micro/10.1099/00221287-127-2-339&mimeType=html&fmt=ahah

References

  1. Alcântara F., Monk M. 1974; Signal propagation during aggregation in the slime mould Dictyostelium discoideum. Journal of General Microbiology 85:321–334
    [Google Scholar]
  2. Chang Y.Y. 1968; Cyclic 3′,5′-adenosine monophosphate phosphodiesterase produced by the slime mold Dictyostelium discoideum. Science 160:57–59
    [Google Scholar]
  3. Devreotes P.N., Steck T.L. 1979; Cyclic 3′,5′-AMP relay in Dictyostelium discoideum. II. Requirements for the initiation and termination of the response. Journal of Cell Biology 80:300–309
    [Google Scholar]
  4. Dicou E.L., Brachet P. 1980; A separate phosphodiesterase for the hydrolysis of cGMP in growing Dictyostelium discoideum amoebae. European Journal of Biochemistry 109:507–514
    [Google Scholar]
  5. Futrelle R.P., McKee W.G., Traut J. 1980; Response of Dictyostelium discoideum to localised cAMP stimuli: computer analysis of cell motion. Journal of Cell Biology 87:57a
    [Google Scholar]
  6. Gerisch G. 1976; Extracellular cAMP phosphodiesterase regulation in agar plate cultures of Dictyostelium discoideum. Cell Differentiation 5:21–25
    [Google Scholar]
  7. Gerisch G., Hess B. 1974; Cyclic AMP controlled oscillations in suspended Dictyosteliumcells: their relation to morphogenetic cell interactions. Proceedings of the National Academy of Sciences of the United States of America 71:2118–2122
    [Google Scholar]
  8. Gerisch G., Wick U. 1975; Intracellular oscillations and release of cyclic AMP from Dictyostelium cells. Biochemical and Biophysical Research Communications 65:364–370
    [Google Scholar]
  9. Gingle A.R., Robertson A. 1976; The development of the relaying competence in Dictyostelium discoideum. Journal of Cell Science 20:21–27
    [Google Scholar]
  10. Green A.A., Newell P.C. 1975; Evidence for the existence of two types of cAMP binding sites in aggregating cells of Dictyostelium discoideum. Cell 6:129–136
    [Google Scholar]
  11. Gross J.D., Peacey M.J., Trevan D.J. 1976; Signal emission and signal propagation during early aggregation in Dictyostelium discoideum. Journal of Cell Science 22:645–656
    [Google Scholar]
  12. Hazelbauer G.L. 1980; Bacterial chemotaxis: molecular biology of a sensory system. Endeavour 4:67–73
    [Google Scholar]
  13. Henderson E.J. 1975; The cyclic adenosine-3′,5′-monophosphate receptor of Dictyostelium discoideum. Journal of Biological Chemistry 250:4730–4736
    [Google Scholar]
  14. Kakebeeke P.J., DeWit R.J.W., Kohtz S.D., Konun T.M. 1979; Negative chemotaxis in Dictyostelium and Polysphondylium. Experimental Cell Research 124:429–434
    [Google Scholar]
  15. Keating M.T., Bonner J.T. 1977; Negative chemotaxis in cellular slime molds. Journal of Bacteriology 130:144–147
    [Google Scholar]
  16. Malchow D., Gerisch G. 1974; Short-term binding and hydrolysis of cyclic 3′,5′-adenosine monophosphate by aggregating Dictyosteliumcells. Proceedings of the National Academy of Sciences of the United States of America 71:2423–2427
    [Google Scholar]
  17. Mato J.M., Konijn T.M. 1977; Chemotactic signal and cGMP accumulation in Dictyostelium. In Development and Differentiation in Cellular Slime Moulds pp. 93–104 Cappuccinelli P., Ashworth J. Edited by Amsterdam:: Elsevier/North Holland.;
    [Google Scholar]
  18. Mato J.M., Marin-Cao D. 1979; Protein and phospholipid methylation during chemotaxis in Dictyostelium discoideum and its relationship to calcium movements. Proceedings of the National Academy of Sciences of the United States of America 76:6106–6109
    [Google Scholar]
  19. Mato J.M., Krens F.A., Van Haastert P.J.M., Konijn T.M. 1977; 3′,5′-cyclic AMP-dependent 3\5′-cyclic GMP accumulation in Dictyostelium discoideum. Proceedings of the National Academy of Sciences of the United States of America 74:2348–2351
    [Google Scholar]
  20. Mato J.M., Woelders H., Konijn T.M. 1979; Intracellular cyclic GMP-binding proteins in cellular slime molds. Journal of Bacteriology 137:169–172
    [Google Scholar]
  21. Mosses D., Williams K.L., Newell P.C. 1975; The use of mitotic crossing over for genetic analysis in Dictyostelium discoideum: mapping of linkage group II. Journal of General Microbiology 90:247–259
    [Google Scholar]
  22. Mullens I.A., Newell P.C. 1978; cAMP binding to cell surface receptors of Dictyostelium. Differentiation 10:171–176
    [Google Scholar]
  23. Pan P., Hall E.M., Bonner J.T. 1975; Determination of the active portion of the folic acid molecule in cellular slime mold chemotaxis. Journal of Bacteriology 122:185–191
    [Google Scholar]
  24. Pannbacker R.G., Bravard L.J. 1972; Phosphodiesterase in Dictyostelium discoideumand the chemotactic response to cyclic adenosine monophosphate. Science 175:1014–1015
    [Google Scholar]
  25. Rahmsdorf H.J., Gerisch G. 1978; Specific binding proteins for cAMP and cGMP in Dictyostelium discoideum. Cell Differentiation 7:249–257
    [Google Scholar]
  26. Raper K.B. 1935; Dictyostelium discoideum, a new species of slime mold from decaying forest leaves. Journal of Agricultural Research 50:135–147
    [Google Scholar]
  27. Robertson A., Drage D.J., Cohen M.H. 1972; Control of aggregation in Dictyostelium discoideum by an external periodic pulse of cyclic adenosine monophosphate. Science 175:333–335
    [Google Scholar]
  28. Roos W., Nanjundiah V., Malchow D., Gerisch G. 1975; Amplification of cyclic-AMP signals in aggregating cells of Dictyostelium discoideum. FEBS Letters 53:139–142
    [Google Scholar]
  29. Ross F.M., Newell P.C. 1979; Genetics of aggregation pattern mutations in the cellular slime mould Dictyostelium discoideum. Journal of General Microbiology 115:289–300
    [Google Scholar]
  30. Shaffer B.M. 1961; The cells founding aggregation centres in the slime mould Polysphondylium violaceum. Journal of Experimental Biology 38:833–849
    [Google Scholar]
  31. Sussman M. 1966; Biochemical and genetic methods in the study of cellular slime mold development. Methods in Cell Physiology 2:397–410
    [Google Scholar]
  32. Williams K.L., Kessin R.H., Newell P.C. 1974; Parasexual genetics in Dictyostelium discoideum: mitotic analysis of acriflavin resistance and growth in axenic medium. Journal of General Microbiology 84:59–69
    [Google Scholar]
  33. Wurster B., Schubiger K., Wick U., Gerisch G. 1977; Cyclic GMP in Dictyostelium discoideum: oscillations and pulses in response to folic acid and cAMP signals. FEBS Letters 76:141–144
    [Google Scholar]
  34. Wurster B., Bozzaro S., Gerisch G. 1978; cGMP regulation and responses of Polysphondylium violaceum to chemoattractants. Cell Biology International Reports 2:61–69
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-127-2-339
Loading
/content/journal/micro/10.1099/00221287-127-2-339
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error