1887

Abstract

The 23 kilobase multiple drug resistance r-determinant (r-det) of the R plasmid NR1 is an IS1-mediated transposon, Tn2671. Drug-resistant transductants isolated after infection with bacteriophage P1::Tn2671 derivatives carry the intact r-det in their chromosomes. Independently isolated transductants carry the r-det at different locations on the chromosome. From the chromosome, Tn2671 can transpose to various locations on the phage P7 genome. Throughout these processes, r-det is maintained as a stable unit. Various possible molecular mechanisms, which all might contribute with characteristic frequencies to the transposition of Tn2671, are discussed. The results presented are relevant to the understanding of mechanisms for a wide spreading of drug resistance genes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-126-2-413
1981-10-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/126/2/mic-126-2-413.html?itemId=/content/journal/micro/10.1099/00221287-126-2-413&mimeType=html&fmt=ahah

References

  1. Adelberg E. A., Burns S. N. 1960; Genetic variations in the sex factor of E. coli. Journal of Bacteriology 79:321–330
    [Google Scholar]
  2. Alton N. K., Vapnek D. 1979; Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9. Nature; London: 282864–869
    [Google Scholar]
  3. Arber W., Iida S., Jütte H., Caspers P., Meyer J., Hänni C. 1978; Rearrangements of genetic material in Escherichia coli as observed on the bacteriophage P1 plasmid. Cold Spring Harbor Symposia on Quantitative Biology 43:1197–1208
    [Google Scholar]
  4. Arthur A., Sherratt D. 1979; Dissection of the transposition process: a transposon-encoded site specific recombination system. Molecular and General Genetics 175:267–274
    [Google Scholar]
  5. Bachmann B. J., Low K. B., Taylor A. L. 1976; Recalibrated linkage map of Escherichia coli. Bacteriological Reviews 40:116–167
    [Google Scholar]
  6. Berg D. E. 1977; Insertion and excision of the transposable kanamycin resistance determinant Tn5. In DNA Insertion Elements, Plasmids, and Episomes pp. 205–212 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  7. Botstein D., Kleckner N. 1977; Transposition and illegitimate recombination by the tetracycline resistance element Tnl0. In DNA Insertion Elements, Plasmids, and Episomes pp. 185–203 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  8. Brevet J., Kopecko D. J., Nisen P., Cohen S. N. 1977; Promotion of insertions and deletions by translocating segments of DNA carrying antibiotic resistance genes. In DNA Insertion Elements, Plasmids, and Episomes pp. 169–178 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  9. Campbell A., Berg D., Botstein D., Lederberg E., Novick P., Starlinger P., Szybalski W. 1977; Nomenclature of transposable elements in prokaryotes. In DNA Insertion Elements, Plasmids, and Episomes pp. 15–22 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  10. Cornelis G., Saedler H. 1980; Deletions and an inversion induced by a resident IS1 of the lactose transposon Tn951. Molecular and General Genetics 178:367–374
    [Google Scholar]
  11. Egawa R., Hirota Y. 1962; Inhibition of fertility by multiple drug resistance (R) factor in Escherichia coli K12. Japanese Journal of Genetics 37:66–69
    [Google Scholar]
  12. Grindley N. D. F., Sherratt D. J. 1978; Sequence analysis at IS1 insertion sites: models for transposition. Cold Spring Harbor Symposia on Quantitative Biology 43:1257–1261
    [Google Scholar]
  13. Heffron F., Rubens C., Falkow S. 1977; Transposition of a plasmid DNA sequence which mediates ampicillin resistance: general description and epidemiological considerations. In DNA Insertion Elements, Plasmids, and Episomes pp. 151–160 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  14. Hu S., Ohtsubo E., Davidson N., Saedler H. 1975; Electron microscope heteroduplex studies of sequence relations among bacterial plasmids: identification and mapping of the insertion sequences IS1 and IS2 in F and R plasmids. Journal of Bacteriology 122:764–775
    [Google Scholar]
  15. Iida S. 1980; A cointegrate of the bacteriophage P1 genome and the conjugative R plasmid R100. Plasmid 3:278–290
    [Google Scholar]
  16. Iida S., Arber W. 1977; Plaque forming specialized transducing phage P1: isolation of P1CmSmSu, a precursor of P1Cm. Molecular and General Genetics 153:259–269
    [Google Scholar]
  17. Iida S., Arber W. 1979; Multiple physical differences in the genome structure of functionally related bacteriophages P1 and P7. Molecular and General Genetics 173:249–261
    [Google Scholar]
  18. Iida S., Arber W. 1980; On the role of IS1 in the formation of hybrids between the bacteriophage P1 and the R plasmid NR1. Molecular and General Genetics 177:261–270
    [Google Scholar]
  19. Iida S., Meyer J., Arber W. 1980a; IS1 mediated inversion observed in phage P1CmTc1 DNA. Experientia 36:748
    [Google Scholar]
  20. Iida S., Meyer J., Arber W. 1980b; Genesis and natural history of IS-mediated transposons. Cold Spring Harbor Symposia on Quantitative Biology 45:27–43
    [Google Scholar]
  21. Iyobe S., Hashimoto H., Mitsuhashi S. 1970; Integration of chloramphenicol resistance genes of an R factor into various sites of an Escherichia coli chromosome. Japanese Journal of Microbiology 14:463–471
    [Google Scholar]
  22. Kleckner N., Chan R. K., Tye B.-K., Botstein D. 1975; Mutagenesis by insertion of a drugresistance element carrying an inverted repetition. Journal of Molecular Biology 97:561–575
    [Google Scholar]
  23. Lane D., Chandler M. 1977; Mapping of the drug resistance genes carried by the r-determinant of the R100-1 plasmid. Molecular and General Genetics 157:17–23
    [Google Scholar]
  24. Lane D., Chandler M., Silver L., Bruschi A., Caro L. 1979; The construction and replication properties of hybrid plasmids composed of the r-determinant of R100-1 and the plasmids pCRl or pSC201. Molecular and General Genetics 168:337–340
    [Google Scholar]
  25. Legrand P., Bouche J.-P., Louarn J.-M. 1979; Direction of deoxyribonucleic acid transfer and replication in a derivative of plasmid R100-1. Journal of Bacteriology 140:1105–1108
    [Google Scholar]
  26. MacHattie L. A., Jackowski J. B. 1977; Physical structure and deletion effects of the chloramphenicol resistance element Tn9 in phage lambda. In DNA Insertion Elements, Plasmids, and Episomes pp. 219–228 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  27. Marcoli R., Iida S., Bickle T. A. 1980; The DNA sequence of an ISl-flanked transposon coding for resistance to chloramphenicol and fusidic acid. FEBS Letters 110:11–14
    [Google Scholar]
  28. Meyer J., Iida S. 1979; Amplification of chloramphenicol resistance transposons carried by phage P1Cm in Escherichia coli. Molecular and General Genetics 176:209–219
    [Google Scholar]
  29. Miki T., Easton M., Rownd R. 1978; Mapping of the resistance genes of the R plasmid NR1. Molecular and General Genetics 158:217–224
    [Google Scholar]
  30. Miller J. H. 1972 Experiments in Molecular Genetics. New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  31. Mise K., Nakaya R. 1977; Transduction of R plasmids by bacteriophages P1 and P22: distinction between generalized and specialized transduction. Molecular and General Genetics 157:131–138
    [Google Scholar]
  32. Novick R. P., Clowes R. C., Cohen S. N., Curtiss R. Jr Datta N., Falkow S. 1976; Uniform nomenclature for bacterial plasmids: a proposal. Bacteriological Reviews 40:168–189
    [Google Scholar]
  33. Ohtsubo E., Zenilman M., Ohtsubo H. 1980; Plasmids containing insertion elements are potential transposons. Proceedings of the National Academy of Sciences of the United States of America 77:750–754
    [Google Scholar]
  34. Ptashne K., Cohen S. N. 1975; Occurrence of insertion sequence (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications. Journal of Bacteriology 122:776–781
    [Google Scholar]
  35. Read H. A., Sarma S. D., Jaskunas S. R. 1980; Fate of donor insertion sequence IS1 during transposition. Proceedings of the National Academy of Sciences of the United States of America 77:2514–2518
    [Google Scholar]
  36. Reif H. J. 1980; Genetic evidence for absence of transposition functions from the internal part of Tn981, a relative of Tn9. Molecular and General Genetics 177:667–674
    [Google Scholar]
  37. Rosner J. L., Guyer M. S. 1980; Transposition of IS1-λ BIO-IS1 from a bacteriophage λ derivative carrying the IS1-cat-IS1 transposon (Tn9). Molecular and General Genetics 178:111–120
    [Google Scholar]
  38. Scott J. R. 1968; Genetic studies on bacteriophage P1. Virology 36:564–574
    [Google Scholar]
  39. Shapiro J. A. 1979; Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements. Proceedings of the National Academy of Sciences of the United States of America 76:1933–1937
    [Google Scholar]
  40. Shapiro J. A., MacHattie L. A. 1978; Integration and excision of prophage λ mediated by the IS1 element. Cold Spring Harbor Symposia on Quantitative Biology 43:1135–1142
    [Google Scholar]
  41. Silver L., Chandler M., Lane H. E. D., Caro L. 1980; Production of extrachromosomal r-determinant circles from integrated R 100-1: involvement of the E. coli recombination system. Molecular and General Genetics 179:565–571
    [Google Scholar]
  42. Southern E. M. 1975; Detection of specificsequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biologv 98:503–517
    [Google Scholar]
  43. Wandersman C., Yarmolinsky M. 1977; Bipartite control of immunity conferred by the related heteroimmune plasmid prophages, P1 and P7 (formerly ϕ Amp). Virology 77:386–400
    [Google Scholar]
  44. Wood W. B. 1966; Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA. Journal of Molecular Biology 16:118–133
    [Google Scholar]
  45. Yarmolinsky M. 1977; Genetic and physical structure of bacteriophage P1 DNA. In DNA Insertion Elements, Plasmids, and Episomes pp. 721–732 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  46. Yun T., Vapnek D. 1977; Electron microscopic analysis of bacteriophages P1, P1Cm and P7. Determination of genome sizes, sequence homology, and location of antibiotic-resistance determinants. Virology 77:376–385
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-126-2-413
Loading
/content/journal/micro/10.1099/00221287-126-2-413
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error