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Motility in Rhizobium leguminosarum WU163 was dependent on the pH of the medium; addition of EDTA or treatment of the medium with a chelating resin was also required. Motility was improved by the addition of Ca2+ but not by Mg2+; both Zn2+ and Cu2+ abolished motility at low concentrations. Chemotaxis was demonstrated to l-arabinose, d-ribose, d-xylose, d-glucose, d-fructose, mannitol, sorbitol, dulcitol, lactose and cellobiose. The organisms were not chemotactic to either sucrose or trehalose under the conditions used.
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