1887

Abstract

Difference spectra at 77 K of intact K12 grown under oxygen-limited conditions revealed the presence of cytochrome . In the presence of CO, the band of the reduced form, observed in both the and regions of the spectrum, was decreased. Dualwavelength scanning spectrophotometry at sub-zero temperatures revealed a flash-dissociable CO-binding pigment with a broad band around 595 nm, identified as cytochrome Photolysis in the presence of O revealed no such band in difference spectra where the reference spectrum was that of the CO-liganded form, a result consistent with the binding of O to cytochrome . Repeated cycles of photolysis and recombination of the cytochrome with CO were demonstrated at −46 °C. The apparent energy of activation for the reaction with CO was 10·9 kcal mol (45·6 kJ mol). The results are discussed in relation to previous assumptions and results regarding ligand binding to cytochrome and the function of this cytochrome in bacterial respiration.

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1981-08-01
2024-10-05
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References

  1. Bashford C. L., Barlow C. H., Chance B., Haselgrove J. 1980; The oxidation-reduction state of cytochrome oxidase in freeze-trapped gerbil brains. FEBS Letters 113:78–80
    [Google Scholar]
  2. Ben-Gershom E. 1961; Use of carbon monoxide for detecting denaturation in haemoproteins. Biochemical Journal 78:218–223
    [Google Scholar]
  3. Bragg P. D. 1979; Electron transport and energy transducing systems of Escherichia coli. In Membrane Proteins in Energy Transduction pp. 341–449 Capaldi R. A. Edited by New York & Basel: Marcel Dekker.;
    [Google Scholar]
  4. Castor L. N., Chance B. 1955; Photochemical action spectra of carbon monoxide-inhibited respiration. Journal of Biological Chemistry 217:453–465
    [Google Scholar]
  5. Castor L. N., Chance B. 1959; Photochemical determinations of the oxidases of bacteria. Journal of Biological Chemistry 234:1587–1592
    [Google Scholar]
  6. Chance B. 1953a; The carbon monoxide compounds of the cytochrome oxidases. I. Difference spectra. Journal of Biological Chemistry 202:383–396
    [Google Scholar]
  7. Chance B. 1953b; The carbon monoxide compounds of the cytochrome oxidases. II. Photodissociation spectra. Journal of Biological Chemistry 202:397–406
    [Google Scholar]
  8. Chance B., Graham N. 1971; A rapid scanning dual wavelength spectrophotometer. Review of Scientific Instruments 42:941–945
    [Google Scholar]
  9. Chance B., Graham N., Legallais V. 1975a; Low temperature trapping method for cytochrome oxidase oxygen intermediates. Analytical Biochemistry 67:552–579
    [Google Scholar]
  10. Chance B., Saronio C., Leigh J. S. 1975b; Functional intermediates in the reaction of membrane-bound cytochrome oxidase with oxygen. Journal of Biological Chemistry 250:9226–9237
    [Google Scholar]
  11. De Fonseka K., Chance B. 1979; Spectroscopic forms of carbonmonoxi-cytochrome oxidase. Biochemical Journal 183:375–379
    [Google Scholar]
  12. Haddock B. A., Downie J. A., Garland P. B. 1976; Kinetic characterization of the membrane-bound cytochromes of Escherichia coli grown under a variety of conditions by using a stopped-flow dual-wavelength spectrophotometer. Biochemical Journal 154:285–294
    [Google Scholar]
  13. Hendler R. W., Nanninga N. 1970; Respiration and protein synthesis in Escherichia coli membrane-envelope fragments. III. Electron microscopy and analysis of the cytochromes. Journal of Cell Biology 46:114–129
    [Google Scholar]
  14. Hendler R. W., Shrager R. I. 1979; Potentiometric analysis of Escherichia coli cytochromes in the optical absorbance range of 500 to 700 nm. Journal of Biological Chemistry 254:11288–11299
    [Google Scholar]
  15. Ingledew W. J. 1977; Cytochrome a1 as an oxidase?. In Functions of Alternative Terminal Oxidases pp. 79–87 Degn H., Lloyd D., Hill G. C. Edited by Oxford: Pergamon Press.;
    [Google Scholar]
  16. Jurtshuk P., Mueller T. J., Acord W. C. 1975; Bacterial terminal oxidases. CRC Critical Reviews in Microbiology 3:399–468
    [Google Scholar]
  17. Kamen M. D., Horio T. 1970; Bacterial cytochromes. I. Structural aspects. Annual Review of Biochemistry 39:673–700
    [Google Scholar]
  18. Keilin D. 1966 The History of Cell Respiration and Cytochrome. Cambridge: Cambridge University Press.;
    [Google Scholar]
  19. Kubowitz F., Haas E. 1932; Ausbau der photochemischen Methoden zur Untersuchung des sauerstoffübertragenden Ferments. (Anwendung auf Essigbakterien und Hefezellen). Biochemische Zeitschrift 255:247–277
    [Google Scholar]
  20. Meyer D. J., Jones C. W. 1973; Reactivity with oxygen of bacterial cytochrome oxidases a1, aa3 and o. FEBS Letters 33:101–105
    [Google Scholar]
  21. Pirt S. J. 1975 Principles of Microbe and Cell Cultivation pp. 90–91 Oxford: Blackwell Scientific Publications.;
    [Google Scholar]
  22. Poole R. K., Chance B. 1980; Intermediates in the reaction with oxygen of cytochrome d in oxygen-limited Escherichia coli. First European Bioenergetics Conference pp. 107–108 Bologna: Pàtron Editore.;
    [Google Scholar]
  23. Poole R. K., Waring A. J., Chance B. 1979a; The reaction of cytochrome o in Escherichia coli with oxygen. Low temperature kinetic and spectral studies. Biochemical Journal 184:379–389
    [Google Scholar]
  24. Poole R. K., Waring A. J., Chance B. 1979b; Evidence for a functional oxygen-bound intermediate in the reaction of Escherichia coli cytochrome o with oxygen. FEBS Letters 101:56–58
    [Google Scholar]
  25. Poole R. K., Scott R. I., Chance B. 1980; Low-temperature spectral and kinetic properties of cytochromes in Escherichia coli grown at lowered oxygen tension. Biochimica et biophysica acta 591:471–482
    [Google Scholar]
  26. Pudek M. R., Bragg P. D. 1974; Inhibition by cyanide of the respiratory chain oxidases of Escherichia coli. Archives of Biochemistry and Biophysics 164:682–693
    [Google Scholar]
  27. Reid G. A., Ingledew W. J. 1979; Characterization and phenotypic control of the cytochrome content of Escherichia coli. Biochemical Journal 182:465–472
    [Google Scholar]
  28. Reid G. A., Ingledew W. J. 1980; The purification of a respiratory oxidase complex from Escherichia coli. FEBS Letters 109:1–4
    [Google Scholar]
  29. Revsin B., Brodie A. F. 1969; Carbon monoxide-binding pigments of Mycobacterium phlei and Escherichia coli. Journal of Biological Chemistry 244:3101–3104
    [Google Scholar]
  30. Rice C. W., Hempfling W. P. 1978; Oxygen-limited continuous culture and respiratory energy conservation in Escherichia coli. Journal of Bacteriology 134:115–124
    [Google Scholar]
  31. Salmon I., Poole R. K. 1980; The mitochondrial cytochromes of an urusual budding yeast, Sterigmatomyces halophilus: spectral characterization exploiting fourth-order finite difference analysis. Journal of General Microbiology 117:315–326
    [Google Scholar]
  32. Scott R. I., Poole R. K., Chance B. 1981; Respiratory biogenesis during the cell cycle of aerobically grown Escherichia coli K12.The accumulation and ligand binding of cytochrome o. Journal of General Microbiology 122:255–261
    [Google Scholar]
  33. Smith L., White D. C., Sinclair P., Chance B. 1970; Rapid reactions of cytochromes of Hemophilus parainfluenzae on addition of substrates or oxygen. Journal of Biological Chemistry 245:5096–5100
    [Google Scholar]
  34. Trutko S. M., Golovchenko N. P., Akhimenko V. K. 1978; Changes in the electron transport chain in Escherichia coli as a function of the conditions of culturing and phase of growth. Mikrobiologiya 47:1–5
    [Google Scholar]
  35. Warburg O., Negelein E., Haas E. 1933; Spirographishämin. Biochemische Zeitschrift 227:171–183
    [Google Scholar]
  36. Weston J. A., Knowles C. J. 1973; A soluble CO-binding c-type cytochrome from the marine bacterium Beneckea natriegens. Biochimica et biophysica acta 305:11–18
    [Google Scholar]
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