1887

Abstract

The DNA-dependent RNA polymerase isolated from 300 contains the following subunits:, polypeptide A and α (molecular weights 149000, 146000, 93000 and 42000, respectively). Polypeptide A is postulated to be the σ factor of the enzyme. The experimental conditions used for the in vitro assay of the enzyme activity, with plasmid pMB9 as template, included: 40 m-Tris/HCl (pH 7.9), 150m-NaCl, 5 m MgCl, at 34 °C. The isolated enzyme was shown by electron microscopy to bind specifically to the region of early promoters of DNA of the bacteriophage T7. Therefore, -specific promoters may have base sequences in common with those of

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1981-08-01
2021-08-05
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