Binding sites of RNA vmerase on RP4 plasmid DNA were determined electron microscopically. Comparison RNA polymerase binding map and the genetic map of RP4 revealed several strong sites outside the well-known RP4 genes. RNA polymerase binding sites for the three tic resistance genes were also detected. Two binding sites were observed for the -on, whereas the and regions showed no prominent affinity for RNA polymer. The genomic regions for the replication origins, (for vegetative replication) and for transfer replication, equivalent to ), both exhibited strong binding to RNA ase, as did genomic regions which code for trans-acting replication functions (B).


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