1887

Abstract

Strains of isogenic except for the locus were grown in glucose-limited chemostats. Anthranilate synthase was assayed spectrofluorimetrically to measure expression. The specific actiyity was about ten times greater in the strain than in the strain. In glucose-limited chemostats, the specific activity of anthranilate synthase was independent of growth rate. Both strains produced two to three times more anthranilate synthase in chemostats than in batch culture. The addition of 19 amino acids (not including tryptophan) increased expression of anthranilate synthase fivefold in the strain.

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/content/journal/micro/10.1099/00221287-123-2-351
1981-04-01
2021-05-14
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References

  1. Botsford J. L., DeMoss R. D. 1971; Catabolite repression of tryptophanase in Escherichia coli . Journal of Bacteriology 105:303–312
    [Google Scholar]
  2. Harman J. G., Botsford J. L. 1979; Synthesis of adenosine 3′:5′-cyclic monophosphate in Salmonella typhimurium growing in continuous culture. Journal of General Microbiology 110:243–246
    [Google Scholar]
  3. Harvey R. J. 1970; Metabolic regulation in glucose- limited chemostat culture of Escherichia coli . Journal of Bacteriology 104:698–706
    [Google Scholar]
  4. Hassan H. M., Fridovich I. 1977; Regulation of superoxide dismutase synthesis in Escherichia coli: glucose effect. Journal of Bacteriology 132:505–510
    [Google Scholar]
  5. Herbert P., Kornberg H. L. 1976; Glucose transport as the rate-limiting step in the growth of Escherichia coli on glucose. Biochemical Journal 156:477–480
    [Google Scholar]
  6. Ito K., Crawford I. P. 1965; Regulation of the enzymes of the tryptophan pathway in Escherichia coli . Genetics 52:1303–1316
    [Google Scholar]
  7. Koch A. L. 1971; The adaptive responses of Escherichia coli to a feast or famine existence. Advances in Microbial Physiology 6:147–217
    [Google Scholar]
  8. Neidhardt F. C., Bloch P. L., Smith D. F. 1974; Culture medium for enterobacteria. Journal of Bacteriology 119:736–747
    [Google Scholar]
  9. Oxender D. L., Zurawski G., Yanofsky C. 1979; Attenuation in the Escherichia coli tryptophan operon: role of RNA secondary structure involving the tryptophan codon region. Proceedings of the National Academy of Sciences of the United States of America 76:5524–5528
    [Google Scholar]
  10. Rose J. K., Yanofsky C. 1972; Metabolic regulation of the tryptophan operon of Escherichia coli: repressor independent regulation of transcription initiation frequency. Journal of Molecular Biology 69:103–118
    [Google Scholar]
  11. Shimizu R. W. 1980 M.S. thesis; New Mexico State University, U.S.A.:
  12. Stubbs J. D., Stubbs E. A. 1971; Influences of various amino acids on tryptophan-mediated control of the tryptophan biosynthetic enzymes in Escherichia coli . Journal of Bacteriology 108:1181–1191
    [Google Scholar]
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