Strains of isogenic except for the locus were grown in glucose-limited chemostats. Anthranilate synthase was assayed spectrofluorimetrically to measure expression. The specific actiyity was about ten times greater in the strain than in the strain. In glucose-limited chemostats, the specific activity of anthranilate synthase was independent of growth rate. Both strains produced two to three times more anthranilate synthase in chemostats than in batch culture. The addition of 19 amino acids (not including tryptophan) increased expression of anthranilate synthase fivefold in the strain.


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