1887

Abstract

Asporogenous and oligosporogenous mutants of blocked at stages III, IV and V and carrying mutations at 18 different genetic loci have been tested for complementation. This was done by preparing protoplasts 3 h after induction of sporulation ( ) and fusing them with wild-type protoplasts prepared in parallel. After incubation to the suspension was assayed for heat-resistant (80 °C, 40 min) colony-forming units. Mutations in 9 of the 18 loci were complemented, i.e. the spore count was increased > 1000-fold. All of the complemented mutants, except for one stage V mutant, were blocked at stage IV. Spo mutants that were complementable by wild-type were also complementable by other Spo mutants provided that they did not carry the same mutation. Complementation analysis was applied to three alleles in the locus. The complementation pattern suggests that there are at least two cistrons in the locus and this agrees with the mapping data.

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/content/journal/micro/10.1099/00221287-123-1-17
1981-03-01
2021-05-06
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References

  1. Audit C., Anagnostopoulos C. 1973; Genetic studies relating to the production of transformed clones diploid in the tryptophan region of the Bacillus subtilis genome. Journal of Bacteriology 114:18–27
    [Google Scholar]
  2. Coote J.G. 1972; Sporulation in Bacillus subtilis. Genetic analysis of oligosporogenous mutants. Journal of General Microbiology 71:17–27
    [Google Scholar]
  3. Ephrati-Elizur E. 1968; Spontaneous transfor- mation in Bacillus subtilis . Genetical Research 11:83–96
    [Google Scholar]
  4. Fitz-James P.C. 1964; Sporulation in protoplasts and its dependence on prior forespore development. Journal of Bacteriology 87:667–675
    [Google Scholar]
  5. Fodor K., Alföldi L. 1976; Fusion of protoplasts of Bacillus megaterium . Proceedings of the National Academy of Sciences of the United States of America 73:2147–2150
    [Google Scholar]
  6. Hopwood D.A., Wright H.M. 1979; Factors affecting recombinant frequency in protoplast fusions of Streptomyces coelicolor. . Journal of General Microbiology 111:137–143
    [Google Scholar]
  7. Hranueli D., Piggot P.J., Mandelstam J. 1974; Statistical estimate of the total number of operons specific for Bacillus subtilis sporulation. Journal of Bacteriology 119:684–690
    [Google Scholar]
  8. Jenkinson H.F., Kay D., Mandelstam J. 1980; . Temporal dissociation of late events in Bacillus subtilis sporulation from expression of genes that determine them. Journal of Bacteriology 141:793–805
    [Google Scholar]
  9. Jenkinson H.F., Sawyer W.D., Mandelstam J. 1981; . Synthesis and order of assembly of spore coat proteins in Bacillus subtilis. . Journal of General Microbiology 123:1–16
    [Google Scholar]
  10. Lencastre H.de, Piggot T. 1979; Identification of different sites of expression for spo loci by transformation of Bacillus subtilis. . Journal of General Microbiology 114:377–389
    [Google Scholar]
  11. Piggot P.J. 1973; Mapping of asporogenous mutations of Bacillus subtilis: a minimum estimate of the number of sporulation operons. Journal of Bacteriology 114:1241–1253
    [Google Scholar]
  12. Piggot P.J. 1978; Organization of spo locus expression during sporulation of Bacillus subtilis: evidence for different loci being expressed in the mother cell and in the forespore. In Spores VII pp. 122–126 Chambliss G., Vary J.C. Edited by Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  13. Piggot P.J., Coote J.G. 1976; Genetic aspects of bacterial endospore formation. Bacteriological Reviews >40:908–962
    [Google Scholar]
  14. Ryter A. 1965; Étude morphologique de la sporulation de Bacillus subtilis. . Annales de l’Institut Pasteur 108:40–60
    [Google Scholar]
  15. Schaeffer P., Cami B., Hotchkiss R.D. 1976; Fusion of bacterial protoplasts. Proceedings of the National Academy of Sciences of the United States of America 73:2151–2155
    [Google Scholar]
  16. Sterlini J.M., Mandelstam J. 1969; Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochemical Journal 113:29–37
    [Google Scholar]
  17. Trowsdale J., Chen S.M.H., Hoch J.A. 1978; Evidence that spoOA mutations are recessive in spo0A/spo0A+ merodiploid strains of Bacillus subtilis. . Journal of Bacteriology 135:99–113
    [Google Scholar]
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