1887

Abstract

A simple method for extraction and analysis of wall-bound mycolic acids from small samples of mycobacteria is described. Separation of mycolic acid classes according to their functional groups by thin-layer chromatography showed a difference between and a number of strains of acid-fast bacilli cultured from leprosy biopsies This technique is proposed as a convenient preliminary test in the identification of possible cultures of

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-121-1-249
1980-11-01
2024-05-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/121/1/mic-121-1-249.html?itemId=/content/journal/micro/10.1099/00221287-121-1-249&mimeType=html&fmt=ahah

References

  1. Bapat C.V., Ranadive K.J., Khanolkar V.R. 1961; Growth characteristics of an acid-fast mycobacterium isolated from human lepromatous leprosy.. International Journal of Leprosy 29:329–342
    [Google Scholar]
  2. Brennan P.J., Souhrada M., Ullom B., Mcclatchy J.K., Goren M.B. 1978; Identification of atypical mycobacteria by thin-layer chromatography of their surface antigens.. Journal of Clinical Microbiology 8:374–379
    [Google Scholar]
  3. Devi S., Stewart-Tull D.E.S. 1979; Detection of mycolic acid in leprous tissue.. International Journal of Leprosy 47:484–486
    [Google Scholar]
  4. Draper P. 1976; Cell walls of Mycobacterium leprae.. International Journal of Leprosy 44:95–98
    [Google Scholar]
  5. Etémadi A.H. 1967; Isomérisation de mycolates de méthyle en milieu alcalin.. Chemistry and Physics of Lipids 1:165–175
    [Google Scholar]
  6. Etémadi A.H., Convit J. 1974; Mycolic acids from ‘non-cultivable’ mycobacteria.. Infection and Immunity 10:236–239
    [Google Scholar]
  7. Goren M.B. 1972; Mycobacterial lipids: selected topics.. Bacteriological Reviews 36:33–64
    [Google Scholar]
  8. Kato L. 1977; The Janus-face of Mycobacterium leprae: characteristics of in vitro grown M. leprae are not predictable.. International Journal of Leprosy 45:175–182
    [Google Scholar]
  9. Kato L., Ishaque M. 1977; In vitro cultivation of mycobacteria from human lepromas and from an armadillo infected with Mycobacterium leprae.. International Journal of Leprosy 45:107–113
    [Google Scholar]
  10. Kubica G.P. 1973; Differential identification of mycobacteria. VII. Key features of identification of clinically significant mycobacteria.. American Review of Respiratory Disease 107:9–21
    [Google Scholar]
  11. Lanéelle M.A., Lanéelle G. 1970; Structures d’acides mycoliques et d’un intermédiaire dans la biosynthese d’acides mycoliques dicarboxy-liques.. European Journal of Biochemistry 12:296–300
    [Google Scholar]
  12. Lederer E. 1971; The mycobacterial cell wall.. Pure and Applied Chemistry 25:135–165
    [Google Scholar]
  13. Mangold H.K. 1969; Aliphatic lipids.. In Thin Layer Chromatography pp. 363–421 Edited by Stahl E. Berlin: Springer-Verlag.;
    [Google Scholar]
  14. Minnikin D.E., Polgar N. 1967a; The mycolic acids from human and avian tubercle bacilli.. Chemical Communications916–918
    [Google Scholar]
  15. Minnikin D.E., Polgar N. 1967b; The methoxy mycolic and ketomycolic acids from human tubercle bacilli.. Chemical Communications1172–1174
    [Google Scholar]
  16. Minnikin D.E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium, Nocardia and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates.. Journal of General Microbiology 88:200–204
    [Google Scholar]
  17. Minnikin D.E., Hutchinson I.G., Caldicott A.B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria.. Journal of Chromatography 188:221–233
    [Google Scholar]
  18. Mordarska H., Mordarski M., Goodfellow M. 1972; Chemotaxonomic characters and classification of some nocardioform bacteria.. Journal of General Microbiology 71:77–86
    [Google Scholar]
  19. Mustafa A.S., Talwar G.P. 1978; Five cultivable mycobacterial strains giving blast transformation and leukocyte migration inhibition of leukocytes analogous to Mycobacterium leprae.. Leprosy in India 50:498–508
    [Google Scholar]
  20. Pattyn S.R. 1973; The problem of cultivation of Mycobacterium leprae.. Bulletin of the World Health Organization 49:403–410
    [Google Scholar]
  21. Smith D.W., Randall H.M., Maclennan A.P., Putney R.K., Rao S.V. 1960; Detection of specific lipids in mycobacteria by infrared spectroscopy.. Journal of Bacteriology 79:217–229
    [Google Scholar]
  22. Stanford J.L., Bird R.G., Carswell J.W., Draper P., Lowe C., Mcdougall A.C., Mcintyre G., Pattyn S.R., Rees R.J.W. 1977; A study of alleged leprosy bacillus strain HI-75.. International Journal of Leprosy 45:101–106
    [Google Scholar]
  23. Toriyama S., Yano I., Masui M., Kusunose E. 1978; Separation of C50–60 and C70–80 mycolic acid molecular species and their changes by growth temperature in Mycobacterium phlei.. FEBS Letters 95:111–115
    [Google Scholar]
  24. Winder F.G., Collins P.B. 1970; Inhibition by isoniazid of synthesis of mycolic acids in Mycobacterium tuberculosis.. Journal of General Microbiology 63:41–48
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-121-1-249
Loading
/content/journal/micro/10.1099/00221287-121-1-249
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error