1887

Abstract

The depolymerase activity of cell-free extracts of nine species of rumen ciliate protozoa and two mixed protozoal preparations, grown and , towards polygalacturonic acid was examined. The highest activity was found with and while there was none in the spined or spineless forms of and little in On the basis of the rapid drop in viscosity, inhibition by EDTA and the production of u.v.-absorbing material, the enzymes from all active species were designated as endopectate lyases (EC 4.2.2.2) although some polygalacturonase may be present. Neither pectin nor polygalacturonic acid supported the survival or growth of any of the protozoal species tested.

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1980-10-01
2021-08-02
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References

  1. Abou Akkada A.R., Howard B.H. 1961; The biochemistry of rumen protozoa. 5. Decomposition of pectic substances. Biochemical Journal 78:512–517
    [Google Scholar]
  2. Coleman G.S. 1969; The metabolism of starch, maltose, glucose and some other sugars by the rumen ciliate Entodinium caudatum. . Journal of General Microbiology 57:303–332
    [Google Scholar]
  3. Coleman G.S. 1978; Rumen Entodiniomorphid protozoa. In Methods of Cultivating Parasites in Vitro pp. 39–54 Edited by Taylor A. E. R., Baker J.R. London: Academic Press;
    [Google Scholar]
  4. Coleman G.S., Laurie J.I. 1977; The metabolism of starch, glucose, amino acids, purines, pyrimidines and bacteria by the rumen ciliate Polyplastron multivesiculatum. . Journal of General Microbiology 98:29–37
    [Google Scholar]
  5. Coleman G.S., Sandford D.C. 1979; The engulfment and digestion of mixed rumen bacteria and individual bacterial species by single and mixed species of rumen ciliate protozoa grown in vivo. . Journal of Agricultural Science, Cambridge 92:729–742
    [Google Scholar]
  6. Coleman G.S., Laurie J.I., Bailey J.E., Holdgate S.A. 1976; The cultivation of cellulolytic protozoa isolated from the rumen. Journal of General Microbiology 95:144–150
    [Google Scholar]
  7. Eadie J.M. 1962; Inter-relationships between certain rumen ciliate protozoa. Journal of General Microbiology 29:579–588
    [Google Scholar]
  8. Eadie J.M. 1967; Studies on the ecology of certain rumen ciliate protozoa. Journal of General Microbiology 49:175–194
    [Google Scholar]
  9. Hasegawa S., Nagel C.W. 1962; The characterisation of α,β unsaturated digalacturonic acid. Journal of Biological Chemistry 237:619–621
    [Google Scholar]
  10. Hsu E.J., Vaughn R.H. 1969; Production and catabolite repression of the constitutive polygalacturonic acid trans-eliminase of Aeromonas liquefaciens. . Journal of Bacteriology 98:172–181
    [Google Scholar]
  11. Jermyn M.A. 1955; Cellulose and hemicelluloses. In Modern Methods of Plant Analysis 2 pp. 197–225 Edited by Paech K., Tracey M.V. Berlin: Springer-Verlag;
    [Google Scholar]
  12. Lee M., Miller L., Macmillan J.D. 1970; Similarities in the action pattern of exopoly- galacturonate lyase and pectinase from Clostridium multifermentans. . Journal of Bacteriology 103:595–600
    [Google Scholar]
  13. Mah R.A., Hungate R.E. 1965; Physiological studies on the rumen ciliate Ophryoscolex purkynei Stein. Journal of Protozoology 12:131–136
    [Google Scholar]
  14. Macmillan J.D., Vaughn R.H. 1964; Purification and properties of a polygalacturonic acid trans-eliminase produced by Clostridium multifermentans. . Biochemistry 3:564–572
    [Google Scholar]
  15. Nagel C.W., Vaughn R.H. 1961; The degradation of oligogalacturonides by the polygalacturonases of Bacillus polymyxa. . Archives of Biochemistry and Biophysics 94:328–332
    [Google Scholar]
  16. Nagel C.W., Wilson T.M. 1970; Pectic acid lyases of Bacillus polymyxa. . Applied Microbiology 20:374–384
    [Google Scholar]
  17. Nasuno S., Starr M.P. 1967; Polygalacturonic acid tmns-eliminase of Xanthomonas campestris. . Biochemical Journal 104:178–185
    [Google Scholar]
  18. Nelson N. 1944; A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry 153:375–380
    [Google Scholar]
  19. Orpin C.G. 1977; Studies on the defaunation of the ovine rumen using dioctyl sodium sulpho- succinate. Journal of Applied Bacteriology 43:309–318
    [Google Scholar]
  20. Phaff H.J. 1966; α-l,4-Polygalacturonide gly- canohydrolase (endo-polygalacturonase) from Saccharomyces fragilis. . Methods in Enzymology 8:636–641
    [Google Scholar]
  21. Preiss J., Ashwell G. 1963; Polygalacturonic acid metabolism in bacteria. 1. Enzymatic formation of 4-deoxy-L-threo-5-hexosulose uronic acid. Journal of Biological Chemistry 238:1571–1576
    [Google Scholar]
  22. Prins R.A. 1977; Biochemical activities of gut micro-organisms. In Microbial Ecology of the Gut pp. 73–183 Edited by Clarke R. T. J., Bauchop T. New Ycrk: Academic Press;
    [Google Scholar]
  23. Rombouts F.M. 1972; Occurrence and properties of bacterial pectate lyases. Agricultural Research Report 779 Wageningen: Centre for Agricultural Publishing and Documentation;
    [Google Scholar]
  24. Warren L. 1960; Thiobarbituric acid spray reagent for deoxysugars and sialic acids. Nature, London 186:237
    [Google Scholar]
  25. Weineck E. 1934; Die Celluloseverdauung bei den Cilaten des Wiederkäuermagens. Archiv für Protistenkunde 82:169–202
    [Google Scholar]
  26. Wojciechowicz M. 1971; Partial characterisation of pectinolytic enzymes of Bacteroides ruminicola isolated from the rumen of a sheep. Acta microbiologica polonica A3:45–50
    [Google Scholar]
  27. Wojciechowicz M., Ziolecki A. 1979; Pectinolytic enzymes of large rumen treponemes. Applied and Environmental Microbiology 37:136–142
    [Google Scholar]
  28. Wright D.E. 1960; Pectic enzymes in rumen protozoa. Archives of Biochemistry and Biophysics 86:251–254
    [Google Scholar]
  29. Wright D.E. 1961; Bloat in cattle. 19. The metabolism of pectin by rumen microorganisms. New Zealand Journal of Agricultural Research 4:203–215
    [Google Scholar]
  30. Ziolecki A., Tomerska H., Wojciechowicz M. 1972; Pectinolytic activity of rumen streptococci. Acta microbiologica polonica A4:183–188
    [Google Scholar]
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