Acetone precipitates from culture filtrates of three isolates of were resolved by isoelectric focusing on polyacrylamide gels to detect differences in the polygalacturonase and protein patterns. Only a few bands — four in the protein patterns and two in the polygalacturonase patterns — were common to all the isolates. Differences were also detected in polygalacturonase and protein patterns of the same isolate at different ages of culture (7, 14 and 21 d). Identical polygalacturonase patterns were obtained when isoelectric focusing was applied to an acetone precipitate either directly or after further purification by ion-exchange chromatography.


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