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Strains of three anaerobic rumen bacteria, Bacteroides ruminicola, Anaerovibrio lipolytica and Selenomonas ruminantium, were able to use extracellular H2 to reduce fumarate to succinate. Each bacterium possessed membrane-bound hydrogenase and fumarate reductase activity. Membrane-bound cytochrome b was reducible by H2 and oxidizable by fumarate in each bacterium. The apparent K m values for hydrogen of the hydrogenases were 4.5 10−6 m, 1.4 10−5 m and 4.4 10−5 m for B. ruminicola, A. lipolytica and S. ruminantium, respectively. The apparent K m values for fumarate of the fumarate reductases were approximately 1.0 10−4 m for each bacterium.
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