1887

Abstract

The effects of different nitrogen sources on the H-uptake hydrogenase activity were studied in batch-grown and carbon- or sulphate-limited chemostat cultures of Hydrogenase activity was significantly higher in N-fixing than in NH - or NO -dependent cultures under carbon limitation or in early-stationary phase batch growth but was not influenced by the nitrogen source in sulphate-limited cultures. Hydrogenase activity developed nearly two generation times later than nitrogenase activity during the transition from NH - to N-dependent growth in carbon-limited cultures. A possible explanation is that H produced by nitrogenase induced hydrogenase synthesis. Carbon-limited N-fixing cultures possessed higher hydrogenase activity than did sulphate- or O-limited cultures. It appears that carbon substrates or metabolites act as primary repressors of hydrogenase activity and that secondary effectors, such as nitrogen sources or H, act when such repression is removed.

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/content/journal/micro/10.1099/00221287-119-2-313
1980-08-01
2021-05-17
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