A restriction endonuclease cleavage map of the tetracycline resistance plasmid pAB124, originally isolated from , was constructed using ten enzymes. Tetracycline resistance was associated with a 1·95 megadalton (Md) region of pAB124 lying between two RI sites, and this region was circularized to produce a viable tetracycline resistance plasmid (pAB224), with two RI fragments of pAB124 deleted amounting to 0·95 Md. A second plasmid (pAB524) with one RI fragment (0·6 Md) of pAB124 deleted was also constructed. Restriction endonuclease cleavage maps of pAB224 and pAB524 were constructed.


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