SUMMARY: The transposons Tn5, Tn7 and Tn10 and bacteriophage Mu have been used to derive insertion mutations in the gene cluster. A large number of deletion mutants have been derived by imprecise excision of insertion mutations and these deletions have been used to construct a fine-structure map of the cluster. Comparison of this genetic map with a physical map of the cluster derived by Reidel (1979) showed a very good correlation between genetic and physical mapping methods.

A new complementation group, designated , has been identified and mapped between and Polarity studies on the 14 cistrons now identified suggests that they are organized in at least seven transcriptional units and that all the multicistronic units are transcribed in the same direction.


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