1887

Abstract

strains A3 and A3(0) which had been grown at 37°C on nutrient agar medium were applied to a DE-52 cellulose/CC-31 cellulose column and eluted with a linear NaCl gradient in 0·05 -phosphate buffer, pH 6·0. Characteristic elution profiles were observed: the encapsulated, slime-forming strain A3 gave an elution peak with 200 m-NaCl and the non-capsulate, non-slimy strain A3(0) with 90 m-NaCl. These observations may be interpreted in terms of ionic interactions between DEAE-cellulose and components of the cell surface.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-117-1-263
1980-03-01
2021-05-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/117/1/mic-117-1-263.html?itemId=/content/journal/micro/10.1099/00221287-117-1-263&mimeType=html&fmt=ahah

References

  1. Aspinall G. O., Jamieson R. S. P., Wilkinson J. F. 1956; The structure of the extracellular polysaccharide of Aerobacter aerogenes A3 (SI) (Klebsiella Type 54). Journal of the Chemical Society3483–3487
    [Google Scholar]
  2. Conrad H. E., Bamburg J. R., Epley J. D., Kindt T. J. 1966; The structure of the Aerobacter aerogenes A3 (SI) polysaccharide. II. Sequence analysis and hydrolysis studies. Biochemistry 5:2808–2817
    [Google Scholar]
  3. Duguid J. P. 1951; The demonstration of bacterial capsules and slime. Journal of Pathology and Bacteriology 63:673–685
    [Google Scholar]
  4. Hall A. N., Hogg S. D., Phillips G. O. 1976; Gradient elution of Salmonella typhimurium and Escherichia coli strains from a DEAE-cellulose column. Journal of Applied Bacteriology 41:189–192
    [Google Scholar]
  5. Hall A. N., Hogg S. D., Phillips G. O. 1978; Influence of growth phase on the gradient elution of Salmonella typhimurium from DEAE-cellulose. Journal of Applied Bacteriology 44:215–223
    [Google Scholar]
  6. James A. M., List C. F. 1966; Some physical investigations of the behaviour of bacterial surfaces. XII. The effect of fimbriae on the electrophoretic mobility of some capsular and noncapsular bacteria of the coli-aerogenes group. Biochemical et biophysica acta
    [Google Scholar]
  7. Salton M. R. J. 1960; Studies of the bacterial cell wall. VII. Monosaccharide constituents of the walls of Gram-negative bacteria. Biochimica et biophysica acta 45:364–371
    [Google Scholar]
  8. Wilkinson J. F., Duguid J. P., Edmunds P. N. 1954; The distribution of polysaccharide production in Aerobacter and Escherichia strains and its relation to antigenic character. Journal of General Microbiology 11:59–72
    [Google Scholar]
  9. Wilkinson J. F., Dudman W. F., Aspinall G. O. 1955; The extracellular polysaccharide of Aerobacter aerogenes A3 (SI) (Klebsiella Type 54). Biochemical Journal 59:446–451
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-117-1-263
Loading
/content/journal/micro/10.1099/00221287-117-1-263
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error