1887

Abstract

The marine bacterium was grown in batch culture on a glucose/NH /salts medium; growth terminated due to either carbon or nitrogen depletion from the medium. Nitrogen-limited cultures converted part of the excess glucose into glycogen whereas the carbon-limited cultures formed little glycogen. Glycogen-rich cultures survived longer than glycogen-poor cultures during starvation. Little protein was utilized during starvation and RNA was degraded as the primary endogenous source of energy. Glycogen was consumed only when the RNA content had decreased to about a third of the growth value.

The adenine nucleotide content of nitrogen-limited cultures increased at the start of the stationary phase but the energy charge remained at the growth value of 0·9 to 0·95. The maximum size of the adenine nucleotide pool depended on the concentration of glucose remaining in the medium at the start of the stationary phase but a limiting value of about 60 mol ATP (g protein) was attained, compared with 12 to 14 mol ATP (g protein) in exponentially growing cultures. During extended starvation of both glycogen-rich and glycogen-poor clutures, there was a large decrease in adenine nucleotide content, but the energy charge remained above 0·6 even when viability was very low.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-116-2-295
1980-02-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/116/2/mic-116-2-295.html?itemId=/content/journal/micro/10.1099/00221287-116-2-295&mimeType=html&fmt=ahah

References

  1. Andersen K. B., Von Meyenburg K. 1977; Changes of nicotinamide adenine nucleotides and adenylate energy charge as regulatory parameters of the metabolism of Escherichia coli . Journal of Biological Chemistry 252:4151–4156
    [Google Scholar]
  2. Atkinson D. E. 1968; The energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers. Biochemistry 7:4030–4034
    [Google Scholar]
  3. Atkinson D. E. 1978 Cellular Energy Metabolism and its Regulation London: Academic Press;
    [Google Scholar]
  4. Baumann P., Baumann L., Mandel M. 1971; Taxonomy of marine bacteria: the genus Beneckea . Journal of Bacteriology 107:268–294
    [Google Scholar]
  5. Blobel G., Potter Van-R. 1968; Distribution of radioactivity between the acid-soluble pool and pools of RNA in the nuclear, non-sedimentable and ribosome fractions of rat liver after a single injection of labelled orotic acid. Biochimica et biophysica acta 166:48–57
    [Google Scholar]
  6. Carter I. S., Dawes E. A. 1979; Effect of oxygen concentration and growth rate on glucose metabolism, poly-β-hydroxybutyrate biosynthesis and respiration of Azotobacter vinelandii . Journal of General Microbiology 110:393–400
    [Google Scholar]
  7. Chapman A. G., Atkinson D. E. 1977; Adenine nucleotide concentrations and turnover rates. Their correlation with biological activity in bacteria and yeast. Advances in Microbial Physiology 15:253–306
    [Google Scholar]
  8. Chapman A. G., Fall L., Atkinson D. E. 1971; Adenylate energy charge in Escherichia coli during growth and starvation. Journal of Bacteriology 108:1072–1086
    [Google Scholar]
  9. Dawes E. A. 1976; Endogenous metabolism and the survival of starved prokaryotes. Symposia of the Society for General Microbiology 26:19–53
    [Google Scholar]
  10. Dawes E. A., Senior P. J. 1973; The role and regulation of energy reserve polymers in microorganisms. Advances in Microbial Physiology 10:136–266
    [Google Scholar]
  11. Dietzler D. N., Lais C. J., Leckie M. P. 1974; Simultaneous increases of the adenylate energy charge and the rate of glycogen synthesis in nitrogen-starved Escherichia coli W4597(K). Archives of Biochemistry and Biophysics 160:14–25
    [Google Scholar]
  12. Fiske C. H., Subbarow Y. 1925; The colorimetric determination of phosphorus. Journal of Bacteriology 66:375–400
    [Google Scholar]
  13. Hassid W. Z., Abraham S. 1957; Chemical procedures for analysis of polysaccharides. Methods in Enzymology 3:34–50
    [Google Scholar]
  14. Herbert D., Phipps P. J., Strange R. E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5B:209–344
    [Google Scholar]
  15. Knowles C. J. 1977; Microbial metabolic regulation by adenine nucleotide pools. Symposia of the Society for General Microbiology 27:241–283
    [Google Scholar]
  16. Knowles C. J. 1979; Adenine nucleotide pool maintenance during bacterial growth and starvation. In Covalent and Non-Covalent Modulation of Protein Function in the Press Edited by Atkinson D. E.
    [Google Scholar]
  17. Mallette M. F. 1969; Evaluation of growth by physical and chemical means. Methods in Microbiology 1:521–566
    [Google Scholar]
  18. Montague M. D., Dawes E. A. 1974; The survival of Peptococcus prévotii in relation to the adenylate energy charge. Journal of General Microbiology 80:291–299
    [Google Scholar]
  19. Niven D. F., Collins P. A., Knowles C. J. 1977a; Adenylate energy charge during batch culture of Beneckea natriegens . Journal of General Microbiology 98:95–108
    [Google Scholar]
  20. Niven D. F., Collins P. A., Knowles C. J. 1977b; Catabolism of adenosine 5′-monophos- phate by extracts of the marine bacterium Beneckea natriegens . Journal of General Microbiology 100:5–13
    [Google Scholar]
  21. Poole R. K., Salmon I. 1978; The pool sizes of adenine nucleotides in exponentially growing, stationary phase and 2´-deoxyadenosine-syn- chronized cultures of Schizosaccharomyces pombe 972 h . Journal of General Microbiology 106:153–164
    [Google Scholar]
  22. Postgate J. R., Crumpton J. E., Hunter J. R. 1961; The measurement of bacterial viabilities by slide culture. Journal of General Microbiology 24:15–24
    [Google Scholar]
  23. Schramm V. L., Lazorik F. C. 1975; The pathway of adenylate catabolism in Azotobacter vinelandii. Evidence for adenosine monophosphate nucleosidase as the regulatory enzyme. Journal of Biological Chemistry 250:1801–1808
    [Google Scholar]
  24. Schramm V. L., Leung H. 1973; Regulation of adenosine monophosphate levels as a function of adenosine triphosphate and inorganic phosphate. A proposed metabolic role for adenosine monophosphate nucleosidase from Azotobacter vinelandii . Journal of Biological Chemistry 248:8313–8315
    [Google Scholar]
  25. Walker-Simmons M., Atkinson D. E. 1977; Functional capacities and the adenylate energy charge in Escherichia coli under conditions of nutritional stress. Journal of Bacteriology 130:676–683
    [Google Scholar]
  26. Yagil E., Beacham I. R. 1975; Uptake of adenosine 5′-monophosphate by Escherichia coli . Journal of Bacteriology 121:401–405
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-116-2-295
Loading
/content/journal/micro/10.1099/00221287-116-2-295
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error