1887

Abstract

An assay was developed to measure the rate of exopolysaccharide formation by washed non-proliferating suspensions of NCIB 11264 grown under a range of controlled environmental conditions. The specific activities of certain of the enzymes involved in the formation of the sugar nucleotide precursors of polysaccharide biosynthesis were also measured in steady-state populations. The level of enzyme activity did not reflect either the amount of extracellular polysaccharide produced or the rate at which glucose was incorporated into exopolysaccharide, which was dependent on medium composition, environmental factors, and the rate and stage of growth of the organism. The specific activities were not affected by either cultural conditions or the separate addition of actinomycin D and chloramphenicol, indicating a constitutive biosynthetic system.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-116-1-133
1980-01-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/116/1/mic-116-1-133.html?itemId=/content/journal/micro/10.1099/00221287-116-1-133&mimeType=html&fmt=ahah

References

  1. Bernheimer A. W. 1953; Synthesis of type III pneumococcal polysaccharide by suspensions of resting cells. Journal of Experimental Medicine 97:591–600
    [Google Scholar]
  2. Catley B. J. 1972; Pullulan elaboration, an inducible system of Pullularia pullulans . FEBS Letters 20:174–176
    [Google Scholar]
  3. Duguid J. P., Wilkinson J. F. 1953; The influence of cultural conditions on polysaccharide production by Aerobacter aerogenes. Journal of General Microbiology 9:174–189
    [Google Scholar]
  4. Eagon R. G. 1956; Studies on polysaccharide formation by Pseudomonas fluorescens. Canadian Journal of Microbiology 2:673–676
    [Google Scholar]
  5. Glaser L., Kornfeld S. 1966 Methods in Enzymology 8:303–305
    [Google Scholar]
  6. Hahn F. E. 1967 In Antibiotics 1 pp. 308–312 Edited by Gottlieb D., Shaw P. D. New York: Springer;
    [Google Scholar]
  7. Haynes W. G. 1951; Pseudomonas aeruginosa: its characterization and identification. Journal of General Microbiology 5:939–950
    [Google Scholar]
  8. Juni E., Heym G. A. 1961; Capsule resynthesis by decapsulated resting cell suspensions. Bacteriological Proceedings 93:
    [Google Scholar]
  9. Maxwell E. S., Kurahashi K., Kalckar H. M. 1962 Methods in Enzymology 5:174–189
    [Google Scholar]
  10. Moraine R. A., Rogovin P. 1966; Kinetics of polysaccharide B-1459 fermentation. Biotechnology and Bioengineering 8:511–524
    [Google Scholar]
  11. Moraine R. A., Rogovin P. 1973; Kinetics of the xanthan fermentation. Biotechnology and Bioengineering 15:225–237
    [Google Scholar]
  12. Munch-Petersen A. 1962 Methods in Enzymology 5:171–174
    [Google Scholar]
  13. Norval M. 1969 Ph.D. thesis Edinburgh University;
  14. Norval M., Sutherland I. W. 1969; A group of Klebsiella mutants showing temperature dependent polysaccharide synthesis. Journal of General Microbiology 57:369–377
    [Google Scholar]
  15. Norval M., Sutherland I. W. 1973; The production of enzymes involved in exopolysaccharide synthesis in Klebsiella aerogenes Types 1 and 8. European Journal of Biochemistry 35:209–215
    [Google Scholar]
  16. Parsons A. B., Dugan P. R. 1971; Production of extracellular polysaccharide matrix by Zcogloea ramigera. Applied Microbiology 21:657–661
    [Google Scholar]
  17. Stow M., Starkey B. J., Hancock I. C., Baddiley J. 1971; Inhibition by chloramphenicol of glucose transfer in teichoic acid biosynthesis. Nature, New Biology 229:56–57
    [Google Scholar]
  18. Sutherland I. W., Norval M., Poxton I. R. 1971; Transfer of monosaccharides to lipid intermediates in the synthesis of Klebsiella exopolysaccharides. Journal of General Microbiology 68:v
    [Google Scholar]
  19. Unz R. F., Farrah S. R. 1976; Exopolymer production and flocculation by Zoogloea MP6. Applied and Environmental Microbiology 31:623–626
    [Google Scholar]
  20. Wilkinson J. F. 1958; The extracellular polysaccharides of bacteria. Bacteriological Reviews 22:46–73
    [Google Scholar]
  21. Wilkinson J. F., Stark G. H. 1956; The synthesis of polysaccharide by washed suspensions of Klebsiella aerogenes. Proceedings of the Royal Physical Society of Edinburgh 2535–38
    [Google Scholar]
  22. Williams A. G., Wimpenny J. W. T. 1975; Exopolysaccharide formation in non-proliferating suspensions of Pseudomonas PB1. Transactions of the Biochemical Society 3:983–985
    [Google Scholar]
  23. Williams A. G., Wimpenny J. W. T. 1977; Exopolysaccharide production by Pseudomonas NCIB11264 grown in batch culture. Journal of General Microbiology 102:13–21
    [Google Scholar]
  24. Williams A. G., Wimpenny J. W. T. 1978; Exopolysaccharide production by Pseudomonas NCIB11264 grown in continuous culture. Journal of General Microbiology 104:47–57
    [Google Scholar]
  25. Williams A. G., Wimpenny J. W. T., Lawson C. J. 1979; Preliminary studies on the composition and rheological properties of the extracellular polysaccharide synthesized by Pseudomonas PB1 (NCIB 11264). Biochimica et biophysica acta 585:611–619
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-116-1-133
Loading
/content/journal/micro/10.1099/00221287-116-1-133
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error