1887

Abstract

Efficient and reproducible cell lysis and the isolation of plasmid DNA from nine phytopathogenic species is described. One to three plasmids, ranging from 23 to 77 megadaltons, were recovered from each strain, as detected by comparative agarose gel electrophoresis. showed considerable plasmid diversity. Other species, such as C. and C. did not. Bacteriocin production, colony morphology, pigmentation and virulence were not correlated with the presence of plasmids. Sedimentation through neutral and alkaline sucrose gradients and electrophoretic separation on agarose gels gave similar values for plasmid molecular weights. Log-linear alkaline sucrose gradients proved useful for molecular weight determinations and also for the isolation of purified plasmids on a preparative scale.

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1979-12-01
2021-08-05
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