Summary: Efficient and reproducible cell lysis and the isolation of plasmid DNA from nine phytopathogenic species is described. One to three plasmids, ranging from 23 to 77 megadaltons, were recovered from each strain, as detected by comparative agarose gel electrophoresis. showed considerable plasmid diversity. Other species, such as and , did not. Bacteriocin production, colony morphology, pigmentation and virulence were not correlated with the presence of plasmids. Sedimentation through neutral and alkaline sucrose gradients and electrophoretic separation on agarose gels gave similar values for plasmid molecular weights. Log-linear alkaline sucrose gradients proved useful for molecular weight determinations and also for the isolation of purified plasmids on a preparative scale.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error