Full text loading...
The minimal nutritional requirements of Escherichia coli have been quantitatively determined in batch culture for cells with (R+) and without (R−) the R plasmid RP1. In these conditions R+ cells have a greater requirement than R− for several nutrients, particularly Mg2+, K+, Fe2+ and PO4 3−. The maximum growth rate in a simple salts medium was the same for R+ and R− cells. At low concentrations of phosphate, the specific growth rate of R+ cells differed from that found for R− cells. The R plasmid was stable in simple salts medium, irrespective of the nutrient ultimately depleted by growth, but, on storage, R+ cells survived for a shorter time than R− cells.
Article metrics loading...
Full text loading...
References
Data & Media loading...