1887

Abstract

Summary: In amoebae of the ribothymidine (rT) content of tRNA is 0·9 mol%, but decreases progressively during development into spores. To elucidate which nucleosides replace rT at position 54 in developmental tRNA we have characterized ‘vegetative’ and ‘developmental’ tRNAs from the slime mould. Specific tRNAs were separated by two-dimensional gel electrophoresis. During early developmental stages, all tRNA species that could be separated by this method were newly synthesized. A new tRNA with uridine in place of rT and having an electrophoretic mobility similar to ‘vegetative’ tRNA was detected during the early preaggregation stage. This ‘developmental’ tRNA was also extracted from purified polysomes. When development proceeds from preaggregation to postaggregation, tRNAs accumulate with 2′-methylribothymidine in place of rT. We suggest that these developmental tRNAs are important for the synthesis of specific developmental proteins.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-115-1-223
1979-11-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/115/1/mic-115-1-223.html?itemId=/content/journal/micro/10.1099/00221287-115-1-223&mimeType=html&fmt=ahah

References

  1. Albani M., Hoburg A., Kersten H., Wurmbach P., Nierhaus K. H. 1978; On the interaction of m5U deficient tRNAPhe and tRNALys with ribosomes. 12th FEBS Meeting, Dresden, Abstract1658
    [Google Scholar]
  2. Björk G. R., Neidhardt F. C. 1974; Physiological and biochemical studies on the function of 5-methyluridine in the transfer ribonucleic acid of Escherichia coli . Journal of Bacteriology 124:99–111
    [Google Scholar]
  3. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  4. Chen E. Y., Roe B. A. 1978; The nucleotide sequence of rat liver tRNAAsn . Biochemical and Biophysical Research Communications 82:235–246
    [Google Scholar]
  5. Chia L. S. Y., Morris P., Randerath K., Randerath E. 1976; Base composition studies on mitochondrial 4S RNA from rat liver and Morris hepatomas 5132 D and 7777. Biochimica et biophysica acta 425:49–62
    [Google Scholar]
  6. Cocucci S., Sussman M. 1970; RNA in cyto-plasmic and nuclear fractions of cellular slime mold amebas. Journal of Cellular Biology 45:399–407
    [Google Scholar]
  7. Dingermann TH., Schmidt W., Kersten H. 1977; Modified bases in tRNA of Dictyostelium discoideum: alterations in the ribothymidine content during development. FEBS Letters 80:205–208
    [Google Scholar]
  8. Erdmann V. A. 1976; Structure and function of 5S and 5·8S RNA. Progress in Nucleic Acid Research and Molecular Biology 18:45–90
    [Google Scholar]
  9. Fradin A., Gruhl H., Feldmann H. 1975; Mapping of yeast tRNAs by two-dimensional electrophoresis on polyacrylamide gels. FEBS Letters 50:185–189
    [Google Scholar]
  10. Gauss D. H., Grüter F., Sprinzl M. 1979; Compilation of tRNA sequences. Nucleic Acids Research 6:r1–r44
    [Google Scholar]
  11. Gerisch G., Malchow D. 1976; AMP receptors and the control of cell aggregation in Dictyostelium . Advances in Cyclic Nucleotide Research 7:49–68
    [Google Scholar]
  12. Gross H. J., Simsek M., Raba M., Limburg K., Heckman J., Rajbhandary U. L. 1974; 2′-O-Methylribothymidine: a component of rabbit liver lysine. Nucleic Acids Research 1:35–43
    [Google Scholar]
  13. Loomis W. F. 1975 Dictyostelium discoideum, a Developmental System New York: Academic Press;
    [Google Scholar]
  14. Mandel L. R., Borek E. 1963; The biosynthesis of methylated bases in ribonucleic acid. Biochemistry 2:555–560
    [Google Scholar]
  15. Marcu K. B., Dudock B. S. 1976; Effect of ribothymidine in specific eukaryotic tRNAs on their efficiency in in vitro protein synthesis. Nature; London: 261159–162
    [Google Scholar]
  16. Palatnik C. M., Katz E. R., Brenner M. 1977; Isolation and characterization of transfer RNAs from Dictyostelium discoideum during growth and development. Journal of Biological Chemistry 252:694–703
    [Google Scholar]
  17. Randerath E., Yu C. -T., Randerath K. 1972; Base analysis of ribopolynucleotides by chemical tritium labeling. Analytical Biochemistry 48:172–198
    [Google Scholar]
  18. Randerath K., Randerath E., Chia L. S. Y., Nowak B. J. 1974; Base analysis of ribopoly-nucleotides by chemical tritium labeling. Analytical Biochemistry 59:263–271
    [Google Scholar]
  19. Reszelbach R., Greenberg R., Pirtle R., Prasad R., Dudock B. 1977; Isolation and comparison of ribothymidine-lacking tRNAs of fetal, newborn and adult bovine tissues. Biochimica et biophysica acta 475:383–392
    [Google Scholar]
  20. Roe B. A., Tsen H. -Y. 1977; Role of ribothy-midine in mammalian tRNAPhe . Proceedings of the National Academy of Sciences of the United States of America 74:3696–3700
    [Google Scholar]
  21. Roe B. A., Chen E. Y., Tsen H. -Y. 1976; Studies on the ribothymidine content of specific rat and human tRNAs: a postulated role for 5-methylcytosine in the regulation of ribothymidine biosynthesis. Biochemical and Biophysical Research Communications 68:1339–1347
    [Google Scholar]
  22. Rogg H., Wehrli W., Staehelin M. 1969; Isolation of mammalian transfer-RNA. Biochimica et biophysica acta 195:13–15
    [Google Scholar]
  23. Rogg H., Brambilla R., Keith G., Staehelin M. 1976; An improved method for separation and quantitation of modified nucleosides of transfer RNA. Nucleic Acids Research 3:285–295
    [Google Scholar]
  24. Schmidt W., Arnold H. H., Kersten H. 1977a; Tetrahydrofolate-dependent biosynthesis of ribothymidine in transfer ribonucleic acids of gram-positive bacteria. Journal of Bacteriology 129:15–21
    [Google Scholar]
  25. Schmidt W., Kersten H., Schweiger H. J. 1977b; Base analysis of tRNA from Acetabularia mediterranea . In Progress in Acetabularia Research pp. 39–43 Woodcock C. L. F. Edited by New York: Academic Press;
    [Google Scholar]
  26. Schwarz U., Lührmann R., Gassen H. G. 1974; On the mRNA induced conformational change of AA-tRNA exposing the T-U-C-G sequence for binding to the 50S ribosomal subunit. Biochemical and Biophysical Research Communications 56:807–814
    [Google Scholar]
  27. Sharma O. K., Borek E. 1970; Inhibitor of transfer ribonucleic acid methylases in the differ-entiating slime mold Dictyostelium discoideum . Journal of Bacteriology 101:705–708
    [Google Scholar]
  28. Watts D. J., Ashworth J. M. 1970; Growth of myxamoebae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochemical Journal 119:171–174
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-115-1-223
Loading
/content/journal/micro/10.1099/00221287-115-1-223
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error