1887

Abstract

Summary: Large colonies of which were about 700 m thick were frozen in liquid nitrogen and sectioned horizontally. The sections were disrupted and several oxidative enzymes were assayed in the crude unfractionated homogenates. In the top 120 m of the colonies the specific activities of the enzymes were high and characteristic of aerobically adapted cells. Cells nearer the base of colonies had very low enzyme activities.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-114-2-487
1979-10-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/114/2/mic-114-2-487.html?itemId=/content/journal/micro/10.1099/00221287-114-2-487&mimeType=html&fmt=ahah

References

  1. King T.E. 1967; The preparation of succinate dehydrogenase and reconstitution of succinate oxidase. Methods in Enzymology 10:322–331
    [Google Scholar]
  2. Ochoa S. 1955; Isocitric dehydrogenase system from pig heart. Methods in Enzymology 1:699–704
    [Google Scholar]
  3. Pirt S.J. 1967; A kinetic study of the mode of growth of surface colonies of bacteria and fungi. Journal of General Microbiology 47:181–197
    [Google Scholar]
  4. Racker E. 1950; Spectrophotometric measurement of the enzymic formation of fumaric and cis-aconitic acids. Biochimica et biophysica acta 4:211–214
    [Google Scholar]
  5. Singleton P., Sainsbury D. 1978 Dictionary of Microbiology p. 99 Chichester: John Wiley;
    [Google Scholar]
  6. Slater E.C. 1950; The components of the dihydrocozymase oxidase system. Biochemical Journal 46:484–503
    [Google Scholar]
  7. Sutherland I.W. 1972; Bacterial exopolysaccharides. Advances in Microbial Physiology 8:143–208
    [Google Scholar]
  8. Wimpenny J.W.T. 1969; Oxygen and carbon dioxide as regulators of microbial growth and metabolism. Symposia of the Society for General Microbiology 19:161–197
    [Google Scholar]
  9. Wimpenny J.W.T. 1979; The growth and form of bacterial colonies. Journal of General Microbiology 114:483–486
    [Google Scholar]
  10. Wimpenny J.W.T., Lewis M.W.A. 1977; The growth and respiration of bacterial colonies. Journal of General Microbiology 103:9–18
    [Google Scholar]
  11. Wimpenny J.W.T., Necklen D.K. 1971; The redox environment and microbial physiology. I. The transition from anaerobiosis to aerobiosis in continuous cultures of facultative bacteria. Biochimica et biophysica acta 253:352–359
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-114-2-487
Loading
/content/journal/micro/10.1099/00221287-114-2-487
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error