Summary: A substance which at low concentration (less than 0.3 μM) stimulated the formation of akinetes in Kütz. was purified from the centrifugal supernatant fluid of sporulating cultures. High resolution mass spectrometry showed that the chemical formula of the substance is CHOSN. Other peaks of high intensity found at values of 123 and 96 in the mass spectrum were produced by loss of CO from the molecular ion, and by additional loss of HCN. Proton nuclear magnetic resonance spectroscopy of the substance showed a complex of peaks in the region of 7.19 to 7.29 p.p.m. Peaks in the infrared absorption spectrum were attributable to methylene C-H bonds and to C = S and cyclic -CO-NH- groups. The most probable structure consistent with the above findings embodies two fused, five-membered rings, one of which is a lactarn and the other of which has a thioketone group.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error