1887

Abstract

Treatment of whole yeast cells with a mixture of a reducing agent and 1,3--glucanase isolated from Basidiomycete QM806 led to the production of sphaeroplasts from ascomycetes, from some fungi imperfecti, but not from basidiomycetes. Association of 1,3--glucanase with a second enzyme, 1,4--glucanase, from , was required for sphaeroplast release from some, but not all, basidiomycetes and fungi imperfecti. The ability of yeast cells to liberate sphaeroplasts following appropriate enzymic treatment is proposed as a taxonomic criterion for differentiating basidiomycetous from ascomycetous yeasts and for classifying fungi imperfecti yeasts.

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1979-07-01
2024-11-08
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References

  1. Bartnicki-Garcia S. 1968; Cell wall chemistry, morphogenesis and taxonomy of fungi. Annual Review of Microbiology 22:87–108
    [Google Scholar]
  2. Bastide J.M., Trave P., Bastide M. 1971; Étude de la paroi cellulaire de Candida macedoniensis: formation de protoplastes. Annales de l’Institut Pasteur 121:311–324
    [Google Scholar]
  3. Bastide M., Trave P., Bastide J.M. 1973; L’hétérogénéité de structure de la paroi dans le genre Candida a-t-elle une incidence taxonomique?. Annales de Microbiologie (Institut Pasteur) 124A:359–373
    [Google Scholar]
  4. Elinov N.P., Vitovskaya G.A., Kaloshin V.G., Kolotinskaya T.M. 1974; Structural polysaccharides of some species of Rhodotorula and Cryptococcus.. Biokhimiya 39:787–792
    [Google Scholar]
  5. Fell J.W., Stazell A.C., Hunter I.L., Phaff H.J. 1969; Leucosporidium gen.n., the heterobasidiomycetous stage of several yeasts of the genus Candida.. Antonie van Leeuwenhoek 35:433–462
    [Google Scholar]
  6. Gorin P.A.J., Spencer J.F.T. 1970; Proton magnetic resonance spectroscopy. An aid in identification and chemotaxonomy of yeasts. Advances in Applied Microbiology 12:25–89
    [Google Scholar]
  7. Hasegawa S., Nordin J. 1969; Enzymes that hydrolyse fungal cell wall polysaccharides. Journal of Biological Chemistry 244:5460–5470
    [Google Scholar]
  8. Kidby D.K., Davies R. 1970; Invertase and disulphide bridges in the yeast wall. Journal of General Microbiology 61:327–333
    [Google Scholar]
  9. Kreger-Van Rij N.J.W., Veenhuis N. 1971; A comparative study of the cell wall structure of basidiomycetous and related yeasts. Journal of General Microbiology 68:87–95
    [Google Scholar]
  10. Kwon-Chung K.J. 1975; Description of a new genus, Filobasidiella, the perfect state of Cryptococcus neoformans.. Mycologia 67:1197–1200
    [Google Scholar]
  11. Kwon-Chung K.J., Popkin T.J. 1976; Ultrastructure of septal complex in Filobasidiella neoformans ( Cryptococcus neoformans). Journal of Bacteriology 126:524–528
    [Google Scholar]
  12. Lampen J.O. 1968; External enzymes of yeast: their nature and formation. Antonie van Leeuwenhoek 34:1–18
    [Google Scholar]
  13. Mann J.W., Heintz C.E., Macmillan J.D. 1972; Yeast sphaeroplasts formed by cell wall degrading enzymes from Oerskovia sp. Journal of Bacteriology 111:821–824
    [Google Scholar]
  14. Meyer S.A., Phaff H.J. 1969; Deoxyribonucleic acid base composition in yeasts. Journal of Bacteriology 97:52–56
    [Google Scholar]
  15. Nakamura N., Tanabe O. 1963; Studies on an enzyme capable of splitting β-d-(l,6)-glucosidic linkage. Agricultural and Biological Chemistry 27:80–87
    [Google Scholar]
  16. Nakase T., Komagata K. 1968; Taxonomic significance of base composition of yeast DNA. Journal of General and Applied Microbiology 14:345–357
    [Google Scholar]
  17. Nakase T., Komagata K. 1971; DNA base composition of some species of yeasts and yeastlike fungi. Journal of General and Applied Microbiology 17:363–369
    [Google Scholar]
  18. Northcote D.H. 1963; The structure and organisation of the polysaccharides of yeast. Pure and Applied Chemistry 7:669–675
    [Google Scholar]
  19. Phaff H.J. 1970; Discussion of the yeast-like genera belonging to the Sporobolomycetaceae. In The Yeasts. A Taxonomic Study, 2nd edn.. pp. 815–862 Lodder J. Edited by Amsterdam: North Holland Publishing Co.;
    [Google Scholar]
  20. Phaff H.J. 1971; Structure and biosynthesis of the yeast cell envelope. In The Yeasts 2 pp. 135–210 Rose A.H., Harrison J.S. Edited by London & New York: Academic Press.;
    [Google Scholar]
  21. Reese E.T., Mandels M. 1959; β-(l–3)-Glucanases in fungi. Canadian Journal of Microbiology 5:173–185
    [Google Scholar]
  22. Rombouts F.M., Phaff H.J. 1976; Lysis of yeast cell walls. Lytic β-(l,3)-glucanases from Bacillus circulans WL-12. European Journal of Biochemistry 63:121–103
    [Google Scholar]
  23. Slodki M.E., Wickerman L.J., Bandoni R.J. 1966; Extracellular heteropolysaccharides from Cryptococcus and Tremella: a possible taxonomic relationship. Canadian Journal of Microbiology 12:489–494
    [Google Scholar]
  24. Somogyi M. 1952; Notes on sugar determination. Journal of Biological Chemistry 195:19–23
    [Google Scholar]
  25. Stenderup A., Leth Bak A. 1969; DNA homology among perfect and imperfect yeasts. Antonie van Leeuwenhoek 35: suppl. A 13.
    [Google Scholar]
  26. Van Der Walt J.P., Hopsu-Havu U.K. 1976; A colour reaction for the differentiation of asco-mycetous and basidiomycetous yeasts. Antonie van Leeuwenhoek 42:157–163
    [Google Scholar]
  27. Yamamoto S., Kobayashi R., Nagasaki S. 1974; Physicochemical, chemical and enzymatic properties of a crystalline yeast cell lytic enzyme from a Rhizopus mold. Agricultural and Biological Chemistry 38:1563–1573
    [Google Scholar]
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