1887

Abstract

Treatment of whole yeast cells with a mixture of a reducing agent and 1,3--glucanase isolated from Basidiomycete QM806 led to the production of sphaeroplasts from ascomycetes, from some fungi imperfecti, but not from basidiomycetes. Association of 1,3--glucanase with a second enzyme, 1,4--glucanase, from , was required for sphaeroplast release from some, but not all, basidiomycetes and fungi imperfecti. The ability of yeast cells to liberate sphaeroplasts following appropriate enzymic treatment is proposed as a taxonomic criterion for differentiating basidiomycetous from ascomycetous yeasts and for classifying fungi imperfecti yeasts.

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1979-07-01
2021-09-24
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