1887

Abstract

SUMMARY: Good synchronous cultures of the fission yeast can be made by selecting small cells from the Beckman JE-6 elutriator rotor. More important, the rate of leucine incorporation in controls shows far less perturbation than after selection from tube gradients. Elutriation may therefore be the best available method for reducing perturbations in selection synchronized cultures.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-112-2-385
1979-06-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/112/2/mic-112-2-385.html?itemId=/content/journal/micro/10.1099/00221287-112-2-385&mimeType=html&fmt=ahah

References

  1. Gordon C. N., Elliott S. G. 1977; Fractionation of Saccharomyces cerevisiae cell populations by centrifugal elutriation. Journal of Bacteriology 129:97–100
    [Google Scholar]
  2. Lloyd D., John L., Edwards C., Chagla A. H. 1975; Synchronous cultures of micro-organisms: large scale preparation by continuous-flow size selection. Journal of General Microbiology 88:153–158
    [Google Scholar]
  3. Meistrich M. L., Meyn R. E., Barlogie B. 1977; Synchronisation of mouse L-P59 cells by centrifugal elutriation separation. Experimental Cell Research 105:169–177
    [Google Scholar]
  4. Mitchell B. F., Tupper J. T. 1977; Synchronisation of mouse 3T3 and SY40 3T3 cells by way of centrifugal elutriation. Experimental Cell Research 106:351–355
    [Google Scholar]
  5. Mitchison J. M. 1970; Physiological and cytological methods for Schizosaccharomyces pombe . Methods in Cell Physiology 4:131–165
    [Google Scholar]
  6. Mitchison J. M. 1977; Enzyme synthesis during the cell cycle. In Cell Differentiation in Micro-organisms, Plants and Animals pp 377–401 Edited by Nover L., Mothes K. Jena: Gustav Fischer Verlag;
    [Google Scholar]
  7. Mitchison J. M., Carter B. L. A. 1975; Cell cycle analysis. Methods in Cell Biology 9:201–219
    [Google Scholar]
  8. Mitchison J. M., Creanor J. 1969; Linear synthesis of sucrase and phosphatases during the cell cycle of Schizosaccharomyces pombe . Journal of Cell Science 5:373–391
    [Google Scholar]
  9. Nurse P. 1975; Genetic control of cell size at cell division in yeast. Nature, London 256:547–551
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-112-2-385
Loading
/content/journal/micro/10.1099/00221287-112-2-385
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error